Comparing the Frequency of Apoptosis and the Level of Bcl-2 Expression in Freshly Isolated WAS Lymphocytes From Different Patients
. | Mean Apoptotic Fraction of Lymphocytes* . | Mean Bcl-2 Expression in Lymphocytes† . |
---|---|---|
Mean normal | 0.00144 ± 0.00120 | 68.0 ± 19.7 |
Patient no. 1 | 0.00184 ± 0.000372 | 51.12 ± 3.63 |
Patient no. 2 | 0.0112 ± 0.000844 | 6.51 ± 1.20 |
Patient no. 3 | 0.00421 ± 0.000886 | 34.51 ± 2.16 |
Patient no. 4 | 0.0121 ± 0.00247 | 38.31 ± 0.834 |
Patient no. 5 | 0.0327 ± 0.0107 | 12.11 ± 2.73 |
. | Mean Apoptotic Fraction of Lymphocytes* . | Mean Bcl-2 Expression in Lymphocytes† . |
---|---|---|
Mean normal | 0.00144 ± 0.00120 | 68.0 ± 19.7 |
Patient no. 1 | 0.00184 ± 0.000372 | 51.12 ± 3.63 |
Patient no. 2 | 0.0112 ± 0.000844 | 6.51 ± 1.20 |
Patient no. 3 | 0.00421 ± 0.000886 | 34.51 ± 2.16 |
Patient no. 4 | 0.0121 ± 0.00247 | 38.31 ± 0.834 |
Patient no. 5 | 0.0327 ± 0.0107 | 12.11 ± 2.73 |
Mean normal represents the mean value for a group of 8 different normal controls (5 adults and 3 children). Patient no. 1 was analyzed once, patient no. 2 was analyzed 3 times, and all others were analyzed twice in independent experiments with unbalanced numbers of replicates.
Mean apoptotic fraction of lymphocytes was measured immediately after isolation using flow cytometry to quantify cells marked using TUNEL.
Mean Bcl-2 expression in lymphocytes was determined by measuring the MFI of cells stained with fluorescein-conjugated secondary antibody directed against bound anti–Bcl-2 monoclonal antibody.