Table 1.

Comparing the Frequency of Apoptosis and the Level of Bcl-2 Expression in Freshly Isolated WAS Lymphocytes From Different Patients

Mean Apoptotic Fraction of Lymphocytes*Mean Bcl-2 Expression in Lymphocytes
Mean normal 0.00144 ± 0.00120  68.0 ± 19.7  
Patient no. 1 0.00184 ± 0.000372  51.12 ± 3.63  
Patient no. 2 0.0112 ± 0.000844  6.51 ± 1.20  
Patient no. 3 0.00421 ± 0.000886  34.51 ± 2.16  
Patient no. 4 0.0121 ± 0.00247  38.31 ± 0.834  
Patient no. 5 0.0327 ± 0.0107  12.11 ± 2.73 
Mean Apoptotic Fraction of Lymphocytes*Mean Bcl-2 Expression in Lymphocytes
Mean normal 0.00144 ± 0.00120  68.0 ± 19.7  
Patient no. 1 0.00184 ± 0.000372  51.12 ± 3.63  
Patient no. 2 0.0112 ± 0.000844  6.51 ± 1.20  
Patient no. 3 0.00421 ± 0.000886  34.51 ± 2.16  
Patient no. 4 0.0121 ± 0.00247  38.31 ± 0.834  
Patient no. 5 0.0327 ± 0.0107  12.11 ± 2.73 

Mean normal represents the mean value for a group of 8 different normal controls (5 adults and 3 children). Patient no. 1 was analyzed once, patient no. 2 was analyzed 3 times, and all others were analyzed twice in independent experiments with unbalanced numbers of replicates.

*

Mean apoptotic fraction of lymphocytes was measured immediately after isolation using flow cytometry to quantify cells marked using TUNEL.

Mean Bcl-2 expression in lymphocytes was determined by measuring the MFI of cells stained with fluorescein-conjugated secondary antibody directed against bound anti–Bcl-2 monoclonal antibody.

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