Comparative Quantitation of the Number of Transplantable Freshly Purified CD34+ CD38− Cells and Transplantable Ex Vivo–Expanded Human Fetal BM CD34+CD38− Cells From the Same Donors
| BM Samples . | SCID-hu Models . | Cell Sources . | |||||
|---|---|---|---|---|---|---|---|
| Freshly Purified Human Fetal CD34+ CD38− Cells . | CD34+ CD38− Cells From Mixed Lymphoid/Myeloid Wells . | CD34+CD38− Cells From Myeloid Wells . | |||||
| Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | ||
| Donor 21 | thy/liv | 18/20 | 37 ± 11 | 19/20 | 38 ± 10 | 0/10 | NA |
| Bone | 18/20 | 35 ± 15 | 19/20 | 37 ± 12 | 1/10 | 3.6 | |
| Donor 22 | thy/liv | 19/20 | 35 ± 13 | 18/20 | 35 ± 8 | 0/10 | NA |
| Bone | 18/20 | 35 ± 20 | 18/20 | 36 ± 13 | 1/10 | 3.4 | |
| Donor 23 | thy/liv | 19/20 | 32 ± 13 | 19/20 | 36 ± 12 | 0/10 | NA |
| Bone | 17/20 | 32 ± 11 | 15/16 | 35 ± 8 | 1/10 | 5.6 | |
| BM Samples . | SCID-hu Models . | Cell Sources . | |||||
|---|---|---|---|---|---|---|---|
| Freshly Purified Human Fetal CD34+ CD38− Cells . | CD34+ CD38− Cells From Mixed Lymphoid/Myeloid Wells . | CD34+CD38− Cells From Myeloid Wells . | |||||
| Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | ||
| Donor 21 | thy/liv | 18/20 | 37 ± 11 | 19/20 | 38 ± 10 | 0/10 | NA |
| Bone | 18/20 | 35 ± 15 | 19/20 | 37 ± 12 | 1/10 | 3.6 | |
| Donor 22 | thy/liv | 19/20 | 35 ± 13 | 18/20 | 35 ± 8 | 0/10 | NA |
| Bone | 18/20 | 35 ± 20 | 18/20 | 36 ± 13 | 1/10 | 3.4 | |
| Donor 23 | thy/liv | 19/20 | 32 ± 13 | 19/20 | 36 ± 12 | 0/10 | NA |
| Bone | 17/20 | 32 ± 11 | 15/16 | 35 ± 8 | 1/10 | 5.6 | |
See Table 4 for legend. These data are compiled from 3 independent experiments using different donor BM samples.