Effect of Growth Factor Cocktail on the Maintenance and Expansion of Purified Human Fetal BM CD34+CD38− Cells In Vitro
| BM Samples . | Frequency of CD34+ CD38−/ Positive Wells . | Percentage of CD34+ CD38−Cells . | |
|---|---|---|---|
| Myeloid Wells . | Mixed Lymphoid/ Myeloid Wells . | ||
| Donor 21 | 69% (207/300) | 9 ± 2 | 15 ± 2 |
| Donor 22 | 71% (214/300) | 7 ± 2 | 15 ± 3 |
| Donor 23 | 72% (216/300) | 10 ± 3 | 16 ± 2 |
| BM Samples . | Frequency of CD34+ CD38−/ Positive Wells . | Percentage of CD34+ CD38−Cells . | |
|---|---|---|---|
| Myeloid Wells . | Mixed Lymphoid/ Myeloid Wells . | ||
| Donor 21 | 69% (207/300) | 9 ± 2 | 15 ± 2 |
| Donor 22 | 71% (214/300) | 7 ± 2 | 15 ± 3 |
| Donor 23 | 72% (216/300) | 10 ± 3 | 16 ± 2 |
A well is scored as CD34+ CD38−/positive well only if it has >1% of CD34+ CD38−cells in the well. Myeloid well is defined as a well without detectable CD19+ B cells. Numbers in the parentheses indicate the total number of CD34+ CD38−/positive wells divided by the total number of wells analyzed in the experiments. Data for the percentage of CD34+ CD38− cells in the CD34+ CD38−/positive wells are presented as the mean ± SD of the total number of CD34+CD38−/positive wells in the cultures. These data are compiled from 3 independent experiments using different donor BM samples.