Table 6.

Effect of Growth Factor Cocktail on the Differentiation Potential of Purified Human Fetal BM CD34+CD38 Cells In Vitro

BM Samples Frequency of Mixed Lymphoid/ Myeloid Wells Percentage of
CD33+ Cells CD19+ Cells
Donor 21 12% (36/300)  63 ± 8  8 ± 2  
Donor 22 10% (31/300)  66 ± 8  9 ± 4  
Donor 23 11% (34/300)  62 ± 6  8 ± 3 
BM Samples Frequency of Mixed Lymphoid/ Myeloid Wells Percentage of
CD33+ Cells CD19+ Cells
Donor 21 12% (36/300)  63 ± 8  8 ± 2  
Donor 22 10% (31/300)  66 ± 8  9 ± 4  
Donor 23 11% (34/300)  62 ± 6  8 ± 3 

The growth factor cocktail includes IL-3, IL-6, GM-CSF, SCF, and LIF. Cells from individual wells were analyzed by flow cytometry after 5 weeks in culture. See Table 2 for legend. These data are compiled from 3 independent experiments using different donor BM samples.

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