Effect of Growth Factor Cocktail on the Differentiation Potential of Purified Human Fetal BM CD34+CD38− Cells In Vitro
BM Samples . | Frequency of Mixed Lymphoid/ Myeloid Wells . | Percentage of . | |
---|---|---|---|
CD33+ Cells . | CD19+ Cells . | ||
Donor 21 | 12% (36/300) | 63 ± 8 | 8 ± 2 |
Donor 22 | 10% (31/300) | 66 ± 8 | 9 ± 4 |
Donor 23 | 11% (34/300) | 62 ± 6 | 8 ± 3 |
BM Samples . | Frequency of Mixed Lymphoid/ Myeloid Wells . | Percentage of . | |
---|---|---|---|
CD33+ Cells . | CD19+ Cells . | ||
Donor 21 | 12% (36/300) | 63 ± 8 | 8 ± 2 |
Donor 22 | 10% (31/300) | 66 ± 8 | 9 ± 4 |
Donor 23 | 11% (34/300) | 62 ± 6 | 8 ± 3 |
The growth factor cocktail includes IL-3, IL-6, GM-CSF, SCF, and LIF. Cells from individual wells were analyzed by flow cytometry after 5 weeks in culture. See Table 2 for legend. These data are compiled from 3 independent experiments using different donor BM samples.