Table 5.

muLIF Can Facilitate Ex Vivo Expansion of Human Fetal BM CD34+ thy-1+ Cells

Treatments Frequency of CD34+thy-1+/ Positive Wells Percentage of CD34+ thy-1+ Cells
IL-3 + IL-6 + GM-CSF + SCF  0.3% (1/300)  1.2 
IL-3 + IL-6 + GM-CSF + SCF + muLIF 10% (30/300)  15 ± 2 
IL-3 + IL-6 + GM-CSF + SCF + huLIF 12% (35/300)  15 ± 1 
Treatments Frequency of CD34+thy-1+/ Positive Wells Percentage of CD34+ thy-1+ Cells
IL-3 + IL-6 + GM-CSF + SCF  0.3% (1/300)  1.2 
IL-3 + IL-6 + GM-CSF + SCF + muLIF 10% (30/300)  15 ± 2 
IL-3 + IL-6 + GM-CSF + SCF + huLIF 12% (35/300)  15 ± 1 

Cells from individual wells were analyzed by flow cytometry after 5 weeks in culture. See Table 3 for legend.

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