Effects of Combinations of Five Cytokines on the Differentiative Potential of Freshly Purified Human Fetal BM CD34+ thy-1+ Cells In Vitro
| Treatments . | Frequency of Mixed Lymphoid/ Myeloid Wells . | Percentages of . | |
|---|---|---|---|
| CD33+ Cells . | CD19+ Cells . | ||
| Control | 55% (165/300) | 55 ± 5 | 8 ± 2 |
| IL-3 | 51% (152/300) | 45 ± 5 | 10 ± 2 |
| IL-6 | 52% (155/300) | 42 ± 6 | 13 ± 2 |
| GM-CSF | 41% (122/300) | 50 ± 8 | 10 ± 3 |
| SCF | 51% (152/300) | 50 ± 5 | 12 ± 3 |
| LIF | 53% (158/300) | 45 ± 3 | 15 ± 2 |
| LIF + IL-3 + IL-6 | 60% (162/300) | 40 ± 5 | 15 ± 3 |
| LIF + IL-3 + IL-6 + GM-CSF | 58% (173/300) | 55 ± 8 | 13 ± 5 |
| LIF + IL-3 + IL-6 + GM-CSF + SCF | 62% (185/300) | 45 ± 3 | 15 ± 2 |
| Treatments . | Frequency of Mixed Lymphoid/ Myeloid Wells . | Percentages of . | |
|---|---|---|---|
| CD33+ Cells . | CD19+ Cells . | ||
| Control | 55% (165/300) | 55 ± 5 | 8 ± 2 |
| IL-3 | 51% (152/300) | 45 ± 5 | 10 ± 2 |
| IL-6 | 52% (155/300) | 42 ± 6 | 13 ± 2 |
| GM-CSF | 41% (122/300) | 50 ± 8 | 10 ± 3 |
| SCF | 51% (152/300) | 50 ± 5 | 12 ± 3 |
| LIF | 53% (158/300) | 45 ± 3 | 15 ± 2 |
| LIF + IL-3 + IL-6 | 60% (162/300) | 40 ± 5 | 15 ± 3 |
| LIF + IL-3 + IL-6 + GM-CSF | 58% (173/300) | 55 ± 8 | 13 ± 5 |
| LIF + IL-3 + IL-6 + GM-CSF + SCF | 62% (185/300) | 45 ± 3 | 15 ± 2 |
Mixed lymphoid/myeloid wells indicate that both CD19+B cells and CD33+ myeloid cells were detected in the same well. Numbers in parentheses indicate the total number of mixed lymphoid/myeloid wells divided by the total number of wells analyzed in the experiments. Data for the percentages of CD19+ and CD33+ cells in the mixed lymphoid/myeloid wells are presented as the mean ± SD of the total number of mixed lymphoid/myeloid wells in each culture condition.