In Vivo Hematopoietic Reconstitution Rate in the SCID-hu Mice With Limiting Numbers of Freshly Purified Human Fetal BM CD34+ thy-1+ Cells
| BM Samples . | SCID-hu Models . | Cell Doses . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 10,000 . | 3,000 . | 1,000 . | 300 . | ||||||
| Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | ||
| Donor 1 | thy/liv | 14/15 | 50 ± 8 | 3/15 | 12 ± 4 | 1/15 | 3.2 | 0/15 | NA |
| Bone | 13/15 | 41 ± 10 | 3/15 | 9 ± 3 | 1/15 | 2.2 | 0/15 | NA | |
| Donor 2 | thy/liv | 14/15 | 52 ± 6 | 3/15 | 15 ± 8 | 1/15 | 2.9 | 0/15 | NA |
| Bone | 14/15 | 42 ± 8 | 3/15 | 8 ± 4 | 1/15 | 2.3 | 0/15 | NA | |
| Donor 3 | thy/liv | 14/15 | 52 ± 8 | 3/15 | 15 ± 3 | 0/15 | n/a | 0/15 | NA |
| Bone | 13/15 | 41 ± 6 | 3/15 | 10 ± 2 | 1/15 | 1.5 | 0/15 | NA | |
| Donor 4 | thy/liv | 14/15 | 51 ± 8 | 4/15 | 11 ± 2 | 1/15 | 2.8 | 0/15 | NA |
| Bone | 13/15 | 42 ± 6 | 3/15 | 10 ± 2 | 1/15 | 2.1 | 0/15 | NA | |
| BM Samples . | SCID-hu Models . | Cell Doses . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| 10,000 . | 3,000 . | 1,000 . | 300 . | ||||||
| Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | Frequency . | Percentage . | ||
| Donor 1 | thy/liv | 14/15 | 50 ± 8 | 3/15 | 12 ± 4 | 1/15 | 3.2 | 0/15 | NA |
| Bone | 13/15 | 41 ± 10 | 3/15 | 9 ± 3 | 1/15 | 2.2 | 0/15 | NA | |
| Donor 2 | thy/liv | 14/15 | 52 ± 6 | 3/15 | 15 ± 8 | 1/15 | 2.9 | 0/15 | NA |
| Bone | 14/15 | 42 ± 8 | 3/15 | 8 ± 4 | 1/15 | 2.3 | 0/15 | NA | |
| Donor 3 | thy/liv | 14/15 | 52 ± 8 | 3/15 | 15 ± 3 | 0/15 | n/a | 0/15 | NA |
| Bone | 13/15 | 41 ± 6 | 3/15 | 10 ± 2 | 1/15 | 1.5 | 0/15 | NA | |
| Donor 4 | thy/liv | 14/15 | 51 ± 8 | 4/15 | 11 ± 2 | 1/15 | 2.8 | 0/15 | NA |
| Bone | 13/15 | 42 ± 6 | 3/15 | 10 ± 2 | 1/15 | 2.1 | 0/15 | NA | |
These data are compiled from 4 independent experiments using different donor BM samples. The frequency of reconstituted animals is presented as the total number of reconstituted animals divided by the total number of animals used in the experiments. The percentage of donor-derived cells represents the mean ± SD of all reconstituted animals from the same experiments.
Abbreviation: NA, not applicable.