Basic Properties of MEL Clones Transfected With the Heavy Subunit of the Ferritin Gene (H-FT)
| . | WT . | Cl-6 . | Cl-12 . | Cl-16 . |
|---|---|---|---|---|
| H-FT (μg/mg protein)* | 0.20 ± 0.07 | 1.10 ± 0.33‡ | 0.90 ± 0.24‡ | 0.31 ± 0.07 |
| LIP (μmol/L)† | 1.30 ± 0.17 | 0.56 ± 0.14‡ | 0.65 ± 0.10‡ | 1.14 ± 0.13 |
| Total Iron μmol/L (BPS)1-153 | 397 ± 150 | 490 ± 88 | 470 ± 94 | 395 ± 140 |
| Total Iron μmol/L (AtAbs)1-155 | 340 ± 10 | 297 ± 23 | 410 ± 14 | 350 ± 20 |
| GSH + GSSG (mmol/L)1-154 | 25 ± 1 | 20 ± 7 | 17 ± 3 | 21 ± 1 |
| GSH (r.f.u.)# | 23 ± 7 | 21 ± 8 | 24 ± 3 | 22 ± 2 |
| . | WT . | Cl-6 . | Cl-12 . | Cl-16 . |
|---|---|---|---|---|
| H-FT (μg/mg protein)* | 0.20 ± 0.07 | 1.10 ± 0.33‡ | 0.90 ± 0.24‡ | 0.31 ± 0.07 |
| LIP (μmol/L)† | 1.30 ± 0.17 | 0.56 ± 0.14‡ | 0.65 ± 0.10‡ | 1.14 ± 0.13 |
| Total Iron μmol/L (BPS)1-153 | 397 ± 150 | 490 ± 88 | 470 ± 94 | 395 ± 140 |
| Total Iron μmol/L (AtAbs)1-155 | 340 ± 10 | 297 ± 23 | 410 ± 14 | 350 ± 20 |
| GSH + GSSG (mmol/L)1-154 | 25 ± 1 | 20 ± 7 | 17 ± 3 | 21 ± 1 |
| GSH (r.f.u.)# | 23 ± 7 | 21 ± 8 | 24 ± 3 | 22 ± 2 |
The various assays used for assessing the indicated properties of the wild type (WT) and the H-FT transfected are depicted in the Methods. Data of
and
were taken from Picard et al.20
Symbols indicate figures statistically significantly different from those of the WT by paired t-test at P < .05.
Assay based on the colorimetric bathophenanthroline sulfonate (BPS) test done on acid cell extracts in triplicates for n = 2 cell preparations.
Based on atomic absorption (AtAbs) measurements done on acid cell extracts in triplicates for n = 3 cell preparations.
Total cell glutathione (GSH + GSSG) was measured in acid cell extracts by the NADPH-GSHR-DTNB enzymatic test (n = 6 cell preparations of duplicate samples).
#GSH was measured in intact cells in triplicates with the MCB reagent (n = 6 cell preparations).