Amino Acid Sequences of Peptides Produced by Processing of Big ET-1, ET-2, and ET-3 by s-Kell
| Substrate . | HPLC Products . | Amino Acid Sequences . |
|---|---|---|
| Big ET-1 | B, Fig 4 | XSXSSLMDKEXVYFXHLDII (W*) (1-21) |
| C, Fig 4 | VNTPEHVVPYGLG(S)PR(S) (22-38) | |
| Big ET-2 | A, Fig 5 | VNTPEQTAPYGLGNP[P] (22-37) |
| C, Fig 5 | XSXSSWLDKEXVYFX(H)LDII(W*) (1-21) | |
| Big ET-3 | A, Fig 6 | INTPEQTVPYGLSNYRGSFR (22-41) |
| B, Fig 6 | XTXFTYKDKEXVYYX[H]LDII(W*) (1-21) |
| Substrate . | HPLC Products . | Amino Acid Sequences . |
|---|---|---|
| Big ET-1 | B, Fig 4 | XSXSSLMDKEXVYFXHLDII (W*) (1-21) |
| C, Fig 4 | VNTPEHVVPYGLG(S)PR(S) (22-38) | |
| Big ET-2 | A, Fig 5 | VNTPEQTAPYGLGNP[P] (22-37) |
| C, Fig 5 | XSXSSWLDKEXVYFX(H)LDII(W*) (1-21) | |
| Big ET-3 | A, Fig 6 | INTPEQTVPYGLSNYRGSFR (22-41) |
| B, Fig 6 | XTXFTYKDKEXVYYX[H]LDII(W*) (1-21) |
In the sequencing cycles marked by X, amino acids were not detected. These positions are predicted to contain cysteine, but, because the cysteines were not derivatized, they were lost and not detected. The cycles marked in brackets were tentative assignments due to low recovery of the amino acids.
Carboxy-terminal tryptophans that were not detected due to low recoveries.