Presence of HCV RNA in 5 Individuals Without Antibodies to HCV More Than 1 Year
Subject . | Visit . | Time in Months . | EIA 3.0 . | RT-PCR . | bDNA (Meq/mL) . | ||
---|---|---|---|---|---|---|---|
Serum . | Plasma . | PBMCs . | Serum . | ||||
1085 | 1 | −94 | − | +* | NA | NA | 0.254* |
2 | −51 | − | +* | NA | + | <0.2 | |
3 | −47 | − | −* | NA | NA | <0.2 | |
4 | −43 | − | +* | NA | + | <0.2 | |
5 | −39 | − | −* | −* | NA | <0.2 | |
6 | −35 | − | −* | −* | NA | <0.2 | |
7 | −27 | − | −* | −* | NA | <0.2 | |
8 | −23 | − | −* | −* | NA | <0.2 | |
9 | −18 | − | −* | −* | NA | <0.2 | |
10 | −14 | − | −* | −* | − | <0.2 | |
11 | −10 | − | −* | −* | − | <0.2 | |
12 | −6 | − | +* | +* | NA | <0.2 | |
13 | −2 | − | −* | −* | NA | <0.2 | |
14 | +2 | + | +* | NA | NA | <0.2 | |
3059 | 1 | −42 | − | − | NA | NA | <0.2 |
2 | −38 | − | +* | NA | + | 0.439* | |
3 | −34 | − | +* | NA | + | 0.686* | |
4 | −31 | − | −* | NA | + | 0.306* | |
5 | −27 | − | −* | NA | NA | <0.2 | |
6 | −22 | − | −* | NA | NA | <0.2 | |
7 | −18 | − | −* | NA | NA | <0.2 | |
8 | −14 | − | + | NA | + | <0.2 | |
9 | −11 | − | −* | − | NA | <0.2 | |
10 | −6 | − | −* | − | NA | <0.2 | |
11 | −2 | − | +* | +* | + | 0.641* | |
12 | +2 | + | +* | + | NA | 0.390 | |
13 | +6 | + | NT | + | NA | 0.211 | |
14 | +10 | + | NT | + | NA | 1.337 | |
0146 | 1 | −28 | − | +* | NA | NA | <0.2 |
2 | −24 | − | −* | NA | NA | <0.2 | |
3 | −21 | − | −* | NA | NA | <0.2 | |
4 | −15 | − | +* | NA | NA | 0.528* | |
5 | −12 | − | −* | NA | NA | <0.2 | |
6 | −9 | − | −* | NA | + | <0.2 | |
7 | −5 | − | −* | NA | NA | <0.2 | |
8 | −2 | − | +* | NA | + | <0.2 | |
9 | +2 | + | +* | NA | NA | <0.2 | |
10 | +5 | + | −* | NA | NA | <0.2 | |
11 | +9 | + | +* | NA | NA | <0.2 | |
0073 | 1 | −44 | − | − | NA | NA | <0.2 |
2 | −40 | − | − | NA | NA | <0.2 | |
3 | −35 | − | − | NA | NA | <0.2 | |
4 | −31 | − | +* | NA | NA | <0.2 | |
5 | −25 | − | +* | NA | NA | <0.2 | |
6 | −16 | − | −* | NA | NA | <0.2 | |
7 | −11 | − | −* | NA | NA | <0.2 | |
8 | +11 | + | NT | −* | NA | <0.2 | |
9 | +16 | + | NT | −* | NA | <0.2 | |
10 | +21 | + | NT | +* | NA | <0.2 | |
11 | +25 | + | NT | +* | NA | 0.346 | |
1083 | 1 | −16 | − | − | NA | NA | <0.2 |
2 | −13 | − | +* | NA | NA | <0.2 | |
3 | −8 | − | −* | NA | NA | <0.2 | |
4 | −6 | − | −* | NA | NA | <0.2 | |
5 | −3 | − | +* | NA | NA | 0.698* | |
6 | +3 | + | −* | NA | NA | <0.2 | |
7 | +12 | + | +* | NT | NA | 0.286 | |
8 | +16 | + | NT | + | NA | 0.472 |
Subject . | Visit . | Time in Months . | EIA 3.0 . | RT-PCR . | bDNA (Meq/mL) . | ||
---|---|---|---|---|---|---|---|
Serum . | Plasma . | PBMCs . | Serum . | ||||
1085 | 1 | −94 | − | +* | NA | NA | 0.254* |
2 | −51 | − | +* | NA | + | <0.2 | |
3 | −47 | − | −* | NA | NA | <0.2 | |
4 | −43 | − | +* | NA | + | <0.2 | |
5 | −39 | − | −* | −* | NA | <0.2 | |
6 | −35 | − | −* | −* | NA | <0.2 | |
7 | −27 | − | −* | −* | NA | <0.2 | |
8 | −23 | − | −* | −* | NA | <0.2 | |
9 | −18 | − | −* | −* | NA | <0.2 | |
10 | −14 | − | −* | −* | − | <0.2 | |
11 | −10 | − | −* | −* | − | <0.2 | |
12 | −6 | − | +* | +* | NA | <0.2 | |
13 | −2 | − | −* | −* | NA | <0.2 | |
14 | +2 | + | +* | NA | NA | <0.2 | |
3059 | 1 | −42 | − | − | NA | NA | <0.2 |
2 | −38 | − | +* | NA | + | 0.439* | |
3 | −34 | − | +* | NA | + | 0.686* | |
4 | −31 | − | −* | NA | + | 0.306* | |
5 | −27 | − | −* | NA | NA | <0.2 | |
6 | −22 | − | −* | NA | NA | <0.2 | |
7 | −18 | − | −* | NA | NA | <0.2 | |
8 | −14 | − | + | NA | + | <0.2 | |
9 | −11 | − | −* | − | NA | <0.2 | |
10 | −6 | − | −* | − | NA | <0.2 | |
11 | −2 | − | +* | +* | + | 0.641* | |
12 | +2 | + | +* | + | NA | 0.390 | |
13 | +6 | + | NT | + | NA | 0.211 | |
14 | +10 | + | NT | + | NA | 1.337 | |
0146 | 1 | −28 | − | +* | NA | NA | <0.2 |
2 | −24 | − | −* | NA | NA | <0.2 | |
3 | −21 | − | −* | NA | NA | <0.2 | |
4 | −15 | − | +* | NA | NA | 0.528* | |
5 | −12 | − | −* | NA | NA | <0.2 | |
6 | −9 | − | −* | NA | + | <0.2 | |
7 | −5 | − | −* | NA | NA | <0.2 | |
8 | −2 | − | +* | NA | + | <0.2 | |
9 | +2 | + | +* | NA | NA | <0.2 | |
10 | +5 | + | −* | NA | NA | <0.2 | |
11 | +9 | + | +* | NA | NA | <0.2 | |
0073 | 1 | −44 | − | − | NA | NA | <0.2 |
2 | −40 | − | − | NA | NA | <0.2 | |
3 | −35 | − | − | NA | NA | <0.2 | |
4 | −31 | − | +* | NA | NA | <0.2 | |
5 | −25 | − | +* | NA | NA | <0.2 | |
6 | −16 | − | −* | NA | NA | <0.2 | |
7 | −11 | − | −* | NA | NA | <0.2 | |
8 | +11 | + | NT | −* | NA | <0.2 | |
9 | +16 | + | NT | −* | NA | <0.2 | |
10 | +21 | + | NT | +* | NA | <0.2 | |
11 | +25 | + | NT | +* | NA | 0.346 | |
1083 | 1 | −16 | − | − | NA | NA | <0.2 |
2 | −13 | − | +* | NA | NA | <0.2 | |
3 | −8 | − | −* | NA | NA | <0.2 | |
4 | −6 | − | −* | NA | NA | <0.2 | |
5 | −3 | − | +* | NA | NA | 0.698* | |
6 | +3 | + | −* | NA | NA | <0.2 | |
7 | +12 | + | +* | NT | NA | 0.286 | |
8 | +16 | + | NT | + | NA | 0.472 |
Abbreviations: NA, not available; NT, not tested.
Repeated independent extractions from aliquots and amplifications.