Table 1.

Influence of HA on Lymphopoiesis in LTBMC

4-wk Lymphoid LTBMC Cells × 104/CultureCFC/Culture
Control  9 ± 1.8  101 ± 25.5 
+HA’ase  3.6 ± 0.37  26 ± 3.7  
+HA 28.7 ± 3.62  176 ± 25.4 
4-wk Lymphoid LTBMC Cells × 104/CultureCFC/Culture
Control  9 ± 1.8  101 ± 25.5 
+HA’ase  3.6 ± 0.37  26 ± 3.7  
+HA 28.7 ± 3.62  176 ± 25.4 

Lymphoid LTBMC were performed as described.25Nonadherent cells (number of cells indicated in Table 1) and clonogenic cells of control cultures or cultures grown in the presence of HA (100 μg/mL; rooster comb HA from Sigma) or HA’ase (Streptomyces HA’ase, Calbiochem; 0.1 U/mL) were determined weekly. Time-course data similar to those for myeloid LTBMC (Figs 1 and 3) were obtained. The Table lists the numbers at 4 weeks, when the maximal effects were achieved.

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