Effects of the Modification of Either Incubation Medium or Treatment Procedure on CyPB-Mediated Adhesion of Platelets to Collagen
| Treatment . | Absence of CyPB . | Presence of CyPB (50 nmol/L) . |
|---|---|---|
| Control3-150 | 29 ± 3 | 49 ± 5 |
| Modified incubation medium | ||
| Ca2+/Mg2+-depleted medium | 12 ± 4 | 12 ± 3 |
| Citrated plasma | 19 ± 2 | 20 ± 3 |
| Recalcified plasma | 33 ± 5 | 53 ± 4 |
| Modified incubation procedure: | ||
| Pretreatment of platelets | 28 ± 4 | 48 ± 6 |
| Pretreatment of coated collagen | 29 ± 1 | 28 ± 2 |
| Treatment . | Absence of CyPB . | Presence of CyPB (50 nmol/L) . |
|---|---|---|
| Control3-150 | 29 ± 3 | 49 ± 5 |
| Modified incubation medium | ||
| Ca2+/Mg2+-depleted medium | 12 ± 4 | 12 ± 3 |
| Citrated plasma | 19 ± 2 | 20 ± 3 |
| Recalcified plasma | 33 ± 5 | 53 ± 4 |
| Modified incubation procedure: | ||
| Pretreatment of platelets | 28 ± 4 | 48 ± 6 |
| Pretreatment of coated collagen | 29 ± 1 | 28 ± 2 |
Results are expressed as percentages of initially added platelets (1 × 107 platelets per well) remaining associated to the collagen-coated well.
Control corresponds to direct incubation of platelets with coated collagen in complete Tyrode’s buffer supplemented with 0.5% BSA.