Table 1. In Vitro Activation of Clonal T... | American Society of Hematology

Table 1.

In Vitro Activation of Clonal T Cells From Two Patients With Chronic Hypereosinophilia

Culture Conditions^*	IL-2 (pg/mL)			IL-4 (pg/mL)			IL-5 (pg/mL)			IL-13 (pg/mL)			IFN-γ (U/mL)
Culture Conditions^*	P1	P2	Control	P1	P2	Control	P1	P2	Control	P1	P2	Control	P1	P2	Control
None	<20	<20	<20	<20	<20	<20	<20	<20	<20	53	<10	<10	<2	<2	<2
PMA alone	1,800	<20	<20	<20	<20	<20	91	165	<20	247	630	<10	<2	<2	<2
PMA + A23187	338,500	309,000	366,000	3,184	10,350	114	34,250	18,900	119	16,050	14,750	21	160	4	4,700
PMA + anti-CD28	33,500	98,000	52,000	45	49	<20	8,400	12,950	802	14,050	2,900	195	<2	4	89

Culture Conditions^*	IL-2 (pg/mL)			IL-4 (pg/mL)			IL-5 (pg/mL)			IL-13 (pg/mL)			IFN-γ (U/mL)
Culture Conditions^*	P1	P2	Control	P1	P2	Control	P1	P2	Control	P1	P2	Control	P1	P2	Control
None	<20	<20	<20	<20	<20	<20	<20	<20	<20	53	<10	<10	<2	<2	<2
PMA alone	1,800	<20	<20	<20	<20	<20	91	165	<20	247	630	<10	<2	<2	<2
PMA + A23187	338,500	309,000	366,000	3,184	10,350	114	34,250	18,900	119	16,050	14,750	21	160	4	4,700
PMA + anti-CD28	33,500	98,000	52,000	45	49	<20	8,400	12,950	802	14,050	2,900	195	<2	4	89

Purified CD3⁻CD4⁺ cells from patient 1 (P1) and patient 2 (P2) or CD3⁺CD4⁺ cells from a healthy blood donor (control) (5 × 10⁵ cells/mL) were stimulated with PMA (1 ng/mL) alone, or PMA + A23187 calcium ionophore (0.1 μg/mL), or PMA + anti-CD28 MoAb (1 μg/mL). After 48 hours, culture supernatants were harvested and cytokine concentrations were determined by ELISA. Data are from 1 of 3 experiments, which gave similar results.

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