Small Bowel Histopathology After BMT
| Parameter . | Syn + Control (n = 15) . | Allo + Control (n = 9) . | Allo + KGF (−3 to 0, n = 4) . | Allo + KGF (−3 to +5, n = 15) . |
|---|---|---|---|---|
| Architecture | ||||
| Crypt destruction | 2.1 ± 0.2 | 3.6 ± 0.2* | 2.8 ± 0.3 | 2.1 ± 0.2‡ |
| Atrophy | 1.9 ± 0.2 | 3.0 ± 0.4* | 3.0 ± 0.5† | 1.5 ± 0.3‡ |
| LP inflammation | 1.8 ± 0.1 | 3.3 ± 0.2* | 2.8 ± 0.3† | 1.8 ± 0.1‡ |
| Villus blunting | 2.1 ± 0.1 | 3.2 ± 0.3* | 3.5 ± 0.3* | 2.8 ± 0.1 |
| Epithelial cytology | ||||
| Vacuolization | 1.0 ± 0.2 | 2.4 ± 0.2* | 1.8 ± 0.5 | 0.9 ± 0.1‡ |
| Cellular attenuation | 1.6 ± 0.1 | 3.0 ± 0.3* | 3.0 ± 0.4* | 1.9 ± 0.2‡ |
| Lymphocytic infiltrate | 1.8 ± 0.1 | 3.1 ± 0.3* | 2.8 ± 0.3† | 1.7 ± 0.1‡ |
| Loss of brush border | 1.8 ± 0.2 | 2.7 ± 0.3* | 3.3 ± 0.3† | 1.9 ± 0.11-153 |
| Total score | 14.0 ± 1.0 | 24.4 ± 1.2* | 22.8 ± 1.9* | 14.8 ± 1.01-155 |
| Parameter . | Syn + Control (n = 15) . | Allo + Control (n = 9) . | Allo + KGF (−3 to 0, n = 4) . | Allo + KGF (−3 to +5, n = 15) . |
|---|---|---|---|---|
| Architecture | ||||
| Crypt destruction | 2.1 ± 0.2 | 3.6 ± 0.2* | 2.8 ± 0.3 | 2.1 ± 0.2‡ |
| Atrophy | 1.9 ± 0.2 | 3.0 ± 0.4* | 3.0 ± 0.5† | 1.5 ± 0.3‡ |
| LP inflammation | 1.8 ± 0.1 | 3.3 ± 0.2* | 2.8 ± 0.3† | 1.8 ± 0.1‡ |
| Villus blunting | 2.1 ± 0.1 | 3.2 ± 0.3* | 3.5 ± 0.3* | 2.8 ± 0.1 |
| Epithelial cytology | ||||
| Vacuolization | 1.0 ± 0.2 | 2.4 ± 0.2* | 1.8 ± 0.5 | 0.9 ± 0.1‡ |
| Cellular attenuation | 1.6 ± 0.1 | 3.0 ± 0.3* | 3.0 ± 0.4* | 1.9 ± 0.2‡ |
| Lymphocytic infiltrate | 1.8 ± 0.1 | 3.1 ± 0.3* | 2.8 ± 0.3† | 1.7 ± 0.1‡ |
| Loss of brush border | 1.8 ± 0.2 | 2.7 ± 0.3* | 3.3 ± 0.3† | 1.9 ± 0.11-153 |
| Total score | 14.0 ± 1.0 | 24.4 ± 1.2* | 22.8 ± 1.9* | 14.8 ± 1.01-155 |
Parameters of small bowel histopathology 5 days after BMT scored from 0 to 4 depending on extent and severity of injury as described in Materials and Methods. KGF was given from either day −3 to 0 or day −3 to +5. Most parameters of injury are significantly worse in the allogeneic control and KGF (day −3 to 0) groups versus syngeneic control groups (*P < .01,
P < .05). KGF given from day −3 to +5 (but not from day −3 to 0) significantly reduced GVHD injury in allogeneic BMT recipients,
P < .01,
P < .05,
P < .001.
Abbreviation: LP, lamina propria.