Biodistributions of Radiolabeled L19 and D1.3 Antibody Fragments in Tumor-Bearing Mice
| %ID/g . | Kidney . | Spleen . | Lung . | Liver . | Blood . | Tumor . | Femur . | Heart . | Muscle . |
|---|---|---|---|---|---|---|---|---|---|
| Time (h) . | |||||||||
| scFv (L19) | |||||||||
| 0.25 | 26.4 ± 4.7 | 5.4 ± 0.4 | 15.1 ± 5.1 | 5.7 ± 0.6 | 14.4 ± 2.6 | 4.6 ± 1.1 | 3.1 ± 0.6 | 5.7 ± 0.9 | 1.1 ± 0.3 |
| 1 | 19.2 ± 3.9 | 3.8 ± 0.3 | 9.8 ± 0.9 | 2.8 ± 0.3 | 8.3 ± 0.9 | 6.9 ± 2.4 | 3.5 ± 0.6 | 4.0 ± 0.5 | 0.9 ± 0.3 |
| 3 | 8.1 ± 1.6 | 2.0 ± 0.3 | 5.0 ± 1.4 | 1.7 ± 0.02 | 4.3 ± 0.3 | 8.2 ± 4.2 | 2.9 ± 0.6 | 2.1 ± 0.3 | 1.1 ± 0.1 |
| 5 | 4.2 ± 1.1 | 1.8 ± 0.2 | 3.5 ± 0.2 | 1.3 ± 0.3 | 2.1 ± 1.6 | 7.7 ± 2.5 | 2.4 ± 0.4 | 1.5 ± 0.4 | 0.9 ± 0.1 |
| 24 | 0.7 ± 0.1 | 0.4 ± 0.1 | 1.0 ± 0.3 | 0.2 ± 0.04 | 0.4 ± 0.1 | 4.7 ± 0.6 | 1.0 ± 0.06 | 0.21 ± 0.07 | 0.2 ± 0.07 |
| scFv (D1.3) | |||||||||
| 0.25 | 46.5 ± 9.4 | 5.0 ± 0.5 | 6.3 ± 1.0 | 7.8 ± 0.6 | 12.7 ± 0.1 | 4.4 ± 1.4 | 2.6 ± 0.1 | 4.4 ± 0.9 | 1.4 ± 0.3 |
| 1 | 16.5 ± 2.2 | 2.6 ± 0.5 | 4.1 ± 1.1 | 3.1 ± 0.6 | 5.7 ± 0.7 | 3.6 ± 1.0 | 1.7 ± 0.4 | 2.1 ± 1.6 | 1.0 ± 0.3 |
| 3 | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 5 | 4.7 ± 0.6 | 1.8 ± 0.5 | 3.0 ± 0.6 | 2.6 ± 0.6 | 4.6 ± 1.2 | 2.6 ± 1.5 | 1.6 ± 0.5 | 1.8 ± 0.4 | 0.8 ± 0.3 |
| 24 | 1.5 ± 0.5 | 0.6 ± 0.2 | 0.8 ± 0.4 | 1.1 ± 0.3 | 1.1 ± 0.5 | 0.7 ± 0.4 | 0.2 ± 0.3 | 0.4 ± 0.2 | 0.2 ± 0.1 |
| %ID/g . | Kidney . | Spleen . | Lung . | Liver . | Blood . | Tumor . | Femur . | Heart . | Muscle . |
|---|---|---|---|---|---|---|---|---|---|
| Time (h) . | |||||||||
| scFv (L19) | |||||||||
| 0.25 | 26.4 ± 4.7 | 5.4 ± 0.4 | 15.1 ± 5.1 | 5.7 ± 0.6 | 14.4 ± 2.6 | 4.6 ± 1.1 | 3.1 ± 0.6 | 5.7 ± 0.9 | 1.1 ± 0.3 |
| 1 | 19.2 ± 3.9 | 3.8 ± 0.3 | 9.8 ± 0.9 | 2.8 ± 0.3 | 8.3 ± 0.9 | 6.9 ± 2.4 | 3.5 ± 0.6 | 4.0 ± 0.5 | 0.9 ± 0.3 |
| 3 | 8.1 ± 1.6 | 2.0 ± 0.3 | 5.0 ± 1.4 | 1.7 ± 0.02 | 4.3 ± 0.3 | 8.2 ± 4.2 | 2.9 ± 0.6 | 2.1 ± 0.3 | 1.1 ± 0.1 |
| 5 | 4.2 ± 1.1 | 1.8 ± 0.2 | 3.5 ± 0.2 | 1.3 ± 0.3 | 2.1 ± 1.6 | 7.7 ± 2.5 | 2.4 ± 0.4 | 1.5 ± 0.4 | 0.9 ± 0.1 |
| 24 | 0.7 ± 0.1 | 0.4 ± 0.1 | 1.0 ± 0.3 | 0.2 ± 0.04 | 0.4 ± 0.1 | 4.7 ± 0.6 | 1.0 ± 0.06 | 0.21 ± 0.07 | 0.2 ± 0.07 |
| scFv (D1.3) | |||||||||
| 0.25 | 46.5 ± 9.4 | 5.0 ± 0.5 | 6.3 ± 1.0 | 7.8 ± 0.6 | 12.7 ± 0.1 | 4.4 ± 1.4 | 2.6 ± 0.1 | 4.4 ± 0.9 | 1.4 ± 0.3 |
| 1 | 16.5 ± 2.2 | 2.6 ± 0.5 | 4.1 ± 1.1 | 3.1 ± 0.6 | 5.7 ± 0.7 | 3.6 ± 1.0 | 1.7 ± 0.4 | 2.1 ± 1.6 | 1.0 ± 0.3 |
| 3 | ND | ND | ND | ND | ND | ND | ND | ND | ND |
| 5 | 4.7 ± 0.6 | 1.8 ± 0.5 | 3.0 ± 0.6 | 2.6 ± 0.6 | 4.6 ± 1.2 | 2.6 ± 1.5 | 1.6 ± 0.5 | 1.8 ± 0.4 | 0.8 ± 0.3 |
| 24 | 1.5 ± 0.5 | 0.6 ± 0.2 | 0.8 ± 0.4 | 1.1 ± 0.3 | 1.1 ± 0.5 | 0.7 ± 0.4 | 0.2 ± 0.3 | 0.4 ± 0.2 | 0.2 ± 0.1 |
Biodistribution studies were performed as described in Materials and Methods.
Abbreviations: %ID/g, percent of antibody injected dose per gram of tissue; ND, not determined.