The Effect of ATRA on CFU-S Production From Cultured lin−c-kit+Sca-1+Hematopoietic Precursors
| Experiment No. 1 . | Experiment No. 2 . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Day of Culture . | ATRA . | No. of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Weight of Spleen (mg) . | Day of Culture . | ATRA . | No. of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Weight of Spleen (mg) . |
| 0 | ND | ND | ND | ND | ND | 0 | − | 500 | 4.25 ± 0.85 | 30.0 ± 10.0 | 60.8 ± 8.00 |
| 7 | − | 6.90 × 105 | 14.0 ± 2.55 | 9.75 ± 1.25 | 57.1 ± 6.00 | 7 | − | 4.80 × 105 | 21.0 ± 2.65 | 7.00 ± 0.00 | 41.4 ± 11.0 |
| 7 | + | 1.65 × 105 | Confluent | Confluent | 91.1 ± 5.00 | 7 | + | 3.08 × 105 | 242 ± 513-150 | 217 ± 883-150 | 107.6 ± 14.0 |
| 14 | − | 2.26 × 106 | 1.00 ± 0.32 | 03-151 | 24.23-151 | 14 | − | 3.92 × 106 | 2.0 ± 1.15 | N/A | N/A |
| 14 | + | 2.96 × 106 | Confluent | Confluent | 55.5 ± 5.00 | 14 | + | 4.28 × 106 | 1250 ± 1603-150 | 3003-150,3-151 | 60.8 ± 4.00 |
| Experiment No. 1 . | Experiment No. 2 . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Day of Culture . | ATRA . | No. of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Weight of Spleen (mg) . | Day of Culture . | ATRA . | No. of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Weight of Spleen (mg) . |
| 0 | ND | ND | ND | ND | ND | 0 | − | 500 | 4.25 ± 0.85 | 30.0 ± 10.0 | 60.8 ± 8.00 |
| 7 | − | 6.90 × 105 | 14.0 ± 2.55 | 9.75 ± 1.25 | 57.1 ± 6.00 | 7 | − | 4.80 × 105 | 21.0 ± 2.65 | 7.00 ± 0.00 | 41.4 ± 11.0 |
| 7 | + | 1.65 × 105 | Confluent | Confluent | 91.1 ± 5.00 | 7 | + | 3.08 × 105 | 242 ± 513-150 | 217 ± 883-150 | 107.6 ± 14.0 |
| 14 | − | 2.26 × 106 | 1.00 ± 0.32 | 03-151 | 24.23-151 | 14 | − | 3.92 × 106 | 2.0 ± 1.15 | N/A | N/A |
| 14 | + | 2.96 × 106 | Confluent | Confluent | 55.5 ± 5.00 | 14 | + | 4.28 × 106 | 1250 ± 1603-150 | 3003-150,3-151 | 60.8 ± 4.00 |
CFU-S are given as the total CFU-S counted per spleen at day 8 (D8) or day 12 (D12) posttransplant (mean ± SEM, n = 5 experiment no. 1; n = 3 experiment no. 2). CFU-S from uncultured lin−c-kit+Sca-1+ cells (day 0) are from 500 freshly sorted hematopoietic precursors. CFU-S after days 7 and 14 of culture are a measure from all cells that grew in liquid suspension culture from 500 or 1,000 hematopoietic precursors, respectively. The total number of cells injected per mouse is given for each time point. The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L, and 10 ng/mL IL-11, without (−) or with (+) 1 μmol/L ATRA. The weights of the spleens are given as the mean ± SEM for D12 spleens at each time point.
Abbreviations: Confluent, confluent spleen colonies; N/A, not available due to deaths of all mice before day 12; ND, not done.
Because of confluent spleen colonies from ATRA-treated cells, numbers are extrapolated from CFU-S of mice injected with all cells that grew in liquid suspension culture from 20 hematopoietic precursors.
Only 1 mouse survived at this time point.