Flow Cytometric Analysis of lin−c-kit+Sca-1+Cells Cultured With or Without 1 μmol/L ATRA for 7 and 14 Days in Liquid Suspension Culture
| Day of Culture . | ATRA . | Percentage of Cells Expressing the Surface Marker . | TNC (×10−4) . | Absolute Number of Cells Expressing the Surface Marker (×10−4) . | ||||
|---|---|---|---|---|---|---|---|---|
| F4/80 . | Gr-1 . | Sca-1 . | F4/80 . | Gr-1 . | Sca-1 . | |||
| 7 | − | 0.6 | 67.9 | 10.4 | 211.2 | 1.3 | 143.3 | 22.0 |
| 7 | + | 18.7 | 25.9 | 44.7 | 53.4 | 10.0 | 13.8 | 23.9 |
| 14 | − | 0.0 | 96.0 | 0.7 | 61.6 | 0.0 | 59.2 | 0.5 |
| 14 | + | 24.6 | 6.7 | 79.9 | 256.0 | 63.1 | 17.2 | 204.5 |
| Day of Culture . | ATRA . | Percentage of Cells Expressing the Surface Marker . | TNC (×10−4) . | Absolute Number of Cells Expressing the Surface Marker (×10−4) . | ||||
|---|---|---|---|---|---|---|---|---|
| F4/80 . | Gr-1 . | Sca-1 . | F4/80 . | Gr-1 . | Sca-1 . | |||
| 7 | − | 0.6 | 67.9 | 10.4 | 211.2 | 1.3 | 143.3 | 22.0 |
| 7 | + | 18.7 | 25.9 | 44.7 | 53.4 | 10.0 | 13.8 | 23.9 |
| 14 | − | 0.0 | 96.0 | 0.7 | 61.6 | 0.0 | 59.2 | 0.5 |
| 14 | + | 24.6 | 6.7 | 79.9 | 256.0 | 63.1 | 17.2 | 204.5 |
One thousand lin−c-kit+Sca-1+hematopoietic precursors were cultured in liquid suspension in media and cytokines (SCF, IL-6, IL-11, and Flt-3 ligand) without (−) or with (+) 1 μmol/L ATRA for 7 and 14 days. Results are expressed as the percentage and absolute number of cells expressing the monocyte/macrophage marker, F4/80, granulocyte marker, Gr-1, or stem cell-associated antigen, Sca-1, at 7 and 14 days after initiation of liquid suspension culture. Results are shown from one single experiment, but multiple repeat experiments yielded similar results.
Abbreviation: TNC, total nucleated cell count.