Phenotypic Analysis of the CD19+Plasmablastic Population
| . | RP5 . | RP7 . | RP8 . | RP9 . |
|---|---|---|---|---|
| Antigens (Ag) | ||||
| PC Ag | ||||
| CD19 | ++ | + | + | ++ |
| CD38 | +++ | ++ | +++ | ++ |
| CD138 | − | − | − | − |
| cIg | + | ++ | +++ | +++ |
| B cells Ag | ||||
| CD19 | ++ | + | + | ++ |
| CD20 | − | − | ND | − |
| CD21 | − | − | ND | − |
| CD22 | +/− | − | ND | +/− |
| CD23 | − | − | ND | − |
| sIg | + | + | ND | + |
| CD40 | + | + | ND | + |
| CD45 | +++ | +++ | +++ | +++ |
| CD45RA | + | ++ | ND | + |
| CD45RO | − | − | ND | − |
| CD80 | +/− | +/− | − | − |
| CD86 | + | + | + | + |
| Adhesion and activation Ag | ||||
| CD25 | − | − | ND | ND |
| CD28 | + (3%) | − | − | ND |
| CD49e | − | − | − | − |
| CD56 | − | − | ND | − |
| CD95 | + | + | + | + |
| HLA-DR | + | +++ | + | ++ |
| . | RP5 . | RP7 . | RP8 . | RP9 . |
|---|---|---|---|---|
| Antigens (Ag) | ||||
| PC Ag | ||||
| CD19 | ++ | + | + | ++ |
| CD38 | +++ | ++ | +++ | ++ |
| CD138 | − | − | − | − |
| cIg | + | ++ | +++ | +++ |
| B cells Ag | ||||
| CD19 | ++ | + | + | ++ |
| CD20 | − | − | ND | − |
| CD21 | − | − | ND | − |
| CD22 | +/− | − | ND | +/− |
| CD23 | − | − | ND | − |
| sIg | + | + | ND | + |
| CD40 | + | + | ND | + |
| CD45 | +++ | +++ | +++ | +++ |
| CD45RA | + | ++ | ND | + |
| CD45RO | − | − | ND | − |
| CD80 | +/− | +/− | − | − |
| CD86 | + | + | + | + |
| Adhesion and activation Ag | ||||
| CD25 | − | − | ND | ND |
| CD28 | + (3%) | − | − | ND |
| CD49e | − | − | − | − |
| CD56 | − | − | ND | − |
| CD95 | + | + | + | + |
| HLA-DR | + | +++ | + | ++ |
Fluorescence staining and analysis were performed as described in Materials and Methods. The phenotype of plasmablastic cells was defined in double staining with CD19 in the CD138− population (after removal of the CD138+ population).
Abbreviations: cIg, cytoplasmic Ig; sIg, surface Ig; ND, not done.