Immunocytochemical Analysis of PBMC-Derived Adherent Cells Cultured With or Without TCM or RTCM
| Culture Condition . | Average % of Positive Cells (min-max values) . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4 . | CD8 . | CD19 . | CD14 . | CD68 . | CD31 . | CD34 . | CD45 . | CD1a . | VE-Cadherin . | FVIII-RA . | |
| RPMI | 3 | 0 | 0 | 17 | 81.3 | 94 | 0 | 97.6 | 0.75-150 | 35-151 | 0 |
| (0-13) | (0-77) | (60-100) | (90-97) | (96-100) | (0-8) | (0-17) | |||||
| N = 7 | N = 3 | N = 8 | N = 7 | N = 4 | N = 3 | N = 4 | N = 5 | N = 12 | N = 15 | N = 3 | |
| TCM or RTCM | 10 | 0.3 | 0 | 13.6 | 90 | 95 | 0 | 100 | 1.95-150 | 225-151 | 0 |
| (1-70) | (0-1) | (0-60) | (86-95.5) | (91-100) | (100-100) | (0-21) | (0-87) | ||||
| N = 11 | N = 6 | N = 6 | N = 7 | N = 4 | N = 3 | N = 4 | N = 5 | N = 1 | N = 15 | N = 3 | |
| Culture Condition . | Average % of Positive Cells (min-max values) . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4 . | CD8 . | CD19 . | CD14 . | CD68 . | CD31 . | CD34 . | CD45 . | CD1a . | VE-Cadherin . | FVIII-RA . | |
| RPMI | 3 | 0 | 0 | 17 | 81.3 | 94 | 0 | 97.6 | 0.75-150 | 35-151 | 0 |
| (0-13) | (0-77) | (60-100) | (90-97) | (96-100) | (0-8) | (0-17) | |||||
| N = 7 | N = 3 | N = 8 | N = 7 | N = 4 | N = 3 | N = 4 | N = 5 | N = 12 | N = 15 | N = 3 | |
| TCM or RTCM | 10 | 0.3 | 0 | 13.6 | 90 | 95 | 0 | 100 | 1.95-150 | 225-151 | 0 |
| (1-70) | (0-1) | (0-60) | (86-95.5) | (91-100) | (100-100) | (0-21) | (0-87) | ||||
| N = 11 | N = 6 | N = 6 | N = 7 | N = 4 | N = 3 | N = 4 | N = 5 | N = 1 | N = 15 | N = 3 | |
Average percentage of adherent cells positive for CD4, CD8, CD19, CD14, CD68, CD31, CD34, CD45, CD1a, VE-cadherin, and FVIII-RA expression by immunocytochemistry after 7 days of culture with TCM or RTCM or growth medium (RPMI). Values are the average of determinations from 8 KS patients, 4 NKS-AIDS patients, and 5 HIV+individuals. Range of determinations with cells from different patients are indicated in parentheses. The results of each determination are from the counting of at least 3 high power macroscopic fields per slide from 1 or more experiments with cells from the same patients. CD19 staining was also confirmed with anti-CD20 antibodies.
Abbreviation: N, number of patients.
Expressed only by 1 HIV+ individual before and after TCM treatment.
VE-cadherin was expressed in RPMI only by KS patients (average, 7%; N = 8) and this was statistically significant (P = .00963) as determined by analysis of variance. VE-cadherin expression was induced by TCM or RTCM in patients from all groups (asymptomatic [N = 3] from 0% to 1%, NKS-AIDS [N = 4] from 0% to 13%, and KS [N = 8] from 7% to 34%), and the effect of TCM or RTCM was statistically significant (P = .028).