Effect of TCM on HHV-8 DNA Detection in Cultured PBMC
| Patients . | PCR-Positive/Total Cultures (%) . | ||||
|---|---|---|---|---|---|
| −TCM . | +TCM . | Increased Signal . | Conversion to Positivity . | Total Responders . | |
| KS | |||||
| AIDS-KS | 6/10 (60%) | 9/10 (90%) | 4/10 (40%) | 3/10 (30%) | 7/10 (70%) |
| At risk of KS | |||||
| NKS-AIDS | 0/7 (0%) | 2/7 (29%) | 0/6 (0%) | 2/7 (29%) | 2/7 (29%) |
| Total | 6/17 (35%) | 11/17 (65%) | 4/17 (23%) | 5/17 (29%) | 9/17 (53%) |
| Patients . | PCR-Positive/Total Cultures (%) . | ||||
|---|---|---|---|---|---|
| −TCM . | +TCM . | Increased Signal . | Conversion to Positivity . | Total Responders . | |
| KS | |||||
| AIDS-KS | 6/10 (60%) | 9/10 (90%) | 4/10 (40%) | 3/10 (30%) | 7/10 (70%) |
| At risk of KS | |||||
| NKS-AIDS | 0/7 (0%) | 2/7 (29%) | 0/6 (0%) | 2/7 (29%) | 2/7 (29%) |
| Total | 6/17 (35%) | 11/17 (65%) | 4/17 (23%) | 5/17 (29%) | 9/17 (53%) |
PBMC from some of the individuals shown in Table 1 were cultured for 6 to 7 days with RPMI or TCM and the same cell number (105) was analyzed. Responders are defined as those patients whose PBMC showed either an increase in the PCR signal or converted to PCR positivity with TCM. All patients’ DNA were positive for β-globin amplification. The percentage of patients (AIDS-KS plus NKS-AIDS) that responded to treatment with TCM was significantly increased (Mc-Nemar test for matched dychotomous data;P = .025).