Reactivity of Sera From Patients No. 4, 5, and 6 Towards Their Respective Purified Cofactors, Directly Immobilized on ELISA Plates Under Various Experimental Conditions
| Plate/Buffer3-150 . | Patient No. . | Control3-151 . | ||
|---|---|---|---|---|
| 4 . | 5 . | 6 . | ||
| MaxiSorp/ Tween | 0.202 ± 0.070 | 0.178 ± 0.056 | 2.482 ± 0.168 | 0.158 ± 0.088 |
| MaxiSorp/ Gelatin | 0.256 ± 0.092 | 0.176 ± 0.042 | 1.911 ± 0.124 | 0.184 ± 0.073 |
| PolySorp/ Tween | 0.153 ± 0.042 | 0.183 ± 0.089 | 0.986 ± 0.097 | 0.190 ± 0.071 |
| PolySorp/ Gelatin | 0.215 ± 0.036 | 0.204 ± 0.075 | 1.693 ± 0.157 | 0.212 ± 0.093 |
| Plate/Buffer3-150 . | Patient No. . | Control3-151 . | ||
|---|---|---|---|---|
| 4 . | 5 . | 6 . | ||
| MaxiSorp/ Tween | 0.202 ± 0.070 | 0.178 ± 0.056 | 2.482 ± 0.168 | 0.158 ± 0.088 |
| MaxiSorp/ Gelatin | 0.256 ± 0.092 | 0.176 ± 0.042 | 1.911 ± 0.124 | 0.184 ± 0.073 |
| PolySorp/ Tween | 0.153 ± 0.042 | 0.183 ± 0.089 | 0.986 ± 0.097 | 0.190 ± 0.071 |
| PolySorp/ Gelatin | 0.215 ± 0.036 | 0.204 ± 0.075 | 1.693 ± 0.157 | 0.212 ± 0.093 |
Specific absorbance values (after sample blank subtraction) are shown.
PBS buffer containing 0.1% Tween or 0.6% gelatin to block plates and dilute samples.
Control denotes a pool of normal human sera reacted onto no. 6 cofactor; its reactivity towards the other purified preparations was essentially the same.