Forward Primers Developed for PCR and Sequence Analysis of Incomplete Dh-Jh Gene Rearrangements
| Primer Code . | Size of Primer (bp) . | Position in bp* . | Sequence (5′ → 3′) . |
|---|---|---|---|
| Dh1 | 22 | −79 | A(C/T)CCAGGAGGCCCCAGAGC(A/T)CA |
| Dh2 | 24 | −187 | CAGCACTGGGCTCAGAGTCCTCTC |
| Dh3 | 24 | −109 | CCTCCTC(A/C)GGTCAGCCC(C/T)GGACAT |
| Dh4 | 24 | −129 | CCCAGGACGCAGCACC(A/G)CTGTCAA |
| Dh5 | 23 | −245 | ACCCAGCCTCCTGCTGACCAGAG |
| Dh6 | 22 | −144 | CAGGCCCCCCA(A/G)AACCAG(T/G)G(A/T)T |
| Dh7 | 24 | −181 | GGGCTGGGGTCTCCCACGTGTTTT |
| Primer Code . | Size of Primer (bp) . | Position in bp* . | Sequence (5′ → 3′) . |
|---|---|---|---|
| Dh1 | 22 | −79 | A(C/T)CCAGGAGGCCCCAGAGC(A/T)CA |
| Dh2 | 24 | −187 | CAGCACTGGGCTCAGAGTCCTCTC |
| Dh3 | 24 | −109 | CCTCCTC(A/C)GGTCAGCCC(C/T)GGACAT |
| Dh4 | 24 | −129 | CCCAGGACGCAGCACC(A/G)CTGTCAA |
| Dh5 | 23 | −245 | ACCCAGCCTCCTGCTGACCAGAG |
| Dh6 | 22 | −144 | CAGGCCCCCCA(A/G)AACCAG(T/G)G(A/T)T |
| Dh7 | 24 | −181 | GGGCTGGGGTCTCCCACGTGTTTT |
The position of the 5′ end of the primer is indicated upstream (−) relative to the 3′ RSS of the involved Dh gene segment.