Semi-quantitative Endpoint Dilution PCR Analysis of HIV DNA Content in 48-Hour PI HIV-Infected DC Treated (mature DC) or Not Treated (immature DC) With CD40L
| HIV-1 Strain . | Immature DC (n = 4) . | Mature DC (n = 5) . | ||
|---|---|---|---|---|
| Ba-L . | LAI . | Ba-L . | LAI . | |
| Range of endpoint titers | 1-50 | 0-5 | 50-500 | 10-10,000 |
| LOG10 mean endpoint titers ± SD | 0.85 ± 0.61 | <0 | 2.02 ± 0.37 | 2.80 ± 1.17 |
| Geometric mean endpoint titers | 7 | <1 | 105 | 630 |
| HIV-1 Strain . | Immature DC (n = 4) . | Mature DC (n = 5) . | ||
|---|---|---|---|---|
| Ba-L . | LAI . | Ba-L . | LAI . | |
| Range of endpoint titers | 1-50 | 0-5 | 50-500 | 10-10,000 |
| LOG10 mean endpoint titers ± SD | 0.85 ± 0.61 | <0 | 2.02 ± 0.37 | 2.80 ± 1.17 |
| Geometric mean endpoint titers | 7 | <1 | 105 | 630 |
Relative amounts of viral DNA in different samples were estimated by endpoint dilutions of the DC lysates in lysates of HIV− A301 cells. Endpoint titers are expressed as the inverse of the last dilution giving a positive signal. The PCR sensitivity being 1 copy/3 × 104 cells, as determined in parallel experiments by serial dilutions of 8E5/LAV cells (1 copy/cell) in HIV− A301 parental cells (data not shown), one may estimate that the geometric mean endpoint titer of 7 corresponds to at least 2 copies/104 cells, the geometric mean endpoint titer of 105 to at least 35 copies/104 cells, and the geometric mean endpoint titer of 630 to at least 210 copies/104cells.