Table 1. PCR Primers Used for... | American Society of Hematology

Table 1.

PCR Primers Used for Amplification of Exons in the Factor XII Gene

Exon	Primer No.	Sequence (5′-3′)	Nucleotide Numbering^*
Exon I	11	CAA GGA AGT TGC TCC ACT TGG CT	−102 to −79
	12A	AAC AAT CCT GGG ACA ATC CTG GTT C	119 to 144
Exon II	21	GAG AGG AAG CTT CAG ACT AGC AAC AGA	4659 to 4685
	22A	ATA CGG ATC CCC ACC CAA GGG TTC C	4824 to 4848
Exon III	31	TCA GAG AGT GTG TTG TCC CTG CAG TTC	7766 to 7792
	32A	AGC AGG GAT CCT TGG ACA GAA GGA CA	7943 to 7968
	33M^†	AGC CCT GCC ACT TCC CCT TCC GGT	7808 to 7831
Exon IV	41	CCT GGG ATC CAT GTA CCC TGC CTG T	7921 to 7945
	42A	CCA GAA TTC CAG GTG TGT GGG GTC TG	8185 to 8210
Exon V and VI	51	AAC CAA GCT TGG AAA CTT GGA GTA GCA A	8299 to 8326
	62A	TCC TCC CTG GCC GGA TCC CAA CCA	8807 to 8830
Exon VII	71	GAG CAG ATG GTT GGG ATC CGG CCA GGG A	8800 to 8827
	72A	TCT CAT AAG CTTTCC GCA CTC TCC CTC CTC	9106 to 9135
Exon VIII	81	TGC GGA AAG CTT ATG AGA GGG AGG CA	9118 to 9143
	82A	TCG TTG TCC GGG ATC CTG TAG CCA C	9432 to 9458
Exon IX	91	TCC GGG ATC CCG GCG CTC TAA CGG CG	9397 to 9422
	92A	GGT CAG GGA ATT CGG CTG CTC CCG CTT	9778 to 9804
Exon X	101	TAG GAA TTC GGG GGG GAA GGA GGA GCC GA	9664 to 9692
	102A	GTG GAA AGA AGC TTG GGG CGG GAG AAG GTA	10088 to 10117
Exon XI	111	AGG AAG CTT GAA CAC GGG ATT GGG GTT	10139 to 10165
	112A	TCC GGA TCCTCC TGA AGG CGC AAC AG	10462 to 10487
Exon XII	121	CCC GTA AGC TTC CAG CAC GAC CTG GGT	10354 to 10380
	122A	GGA ACG ATA CCG AAT TCG CGG GCT TCT T	10665 to 10692
Exon XIII	131	GAG AAG CTT TGT CCA TCG TCC GGG CGG C	11086 to 11113
	132A	TGT GGA CCT GAG AAT TCT GCC TGA CGG CCT	11341 to 11370
Exon XIV	141	GTG CCA GGT AAG CTT TTA GCC CGG TTG	11301 to 11327
	142A	ACA CAA TCT TGA ATT CCA TGC CCC AGC CA	11615 to 11643

Exon	Primer No.	Sequence (5′-3′)	Nucleotide Numbering^*
Exon I	11	CAA GGA AGT TGC TCC ACT TGG CT	−102 to −79
	12A	AAC AAT CCT GGG ACA ATC CTG GTT C	119 to 144
Exon II	21	GAG AGG AAG CTT CAG ACT AGC AAC AGA	4659 to 4685
	22A	ATA CGG ATC CCC ACC CAA GGG TTC C	4824 to 4848
Exon III	31	TCA GAG AGT GTG TTG TCC CTG CAG TTC	7766 to 7792
	32A	AGC AGG GAT CCT TGG ACA GAA GGA CA	7943 to 7968
	33M^†	AGC CCT GCC ACT TCC CCT TCC GGT	7808 to 7831
Exon IV	41	CCT GGG ATC CAT GTA CCC TGC CTG T	7921 to 7945
	42A	CCA GAA TTC CAG GTG TGT GGG GTC TG	8185 to 8210
Exon V and VI	51	AAC CAA GCT TGG AAA CTT GGA GTA GCA A	8299 to 8326
	62A	TCC TCC CTG GCC GGA TCC CAA CCA	8807 to 8830
Exon VII	71	GAG CAG ATG GTT GGG ATC CGG CCA GGG A	8800 to 8827
	72A	TCT CAT AAG CTTTCC GCA CTC TCC CTC CTC	9106 to 9135
Exon VIII	81	TGC GGA AAG CTT ATG AGA GGG AGG CA	9118 to 9143
	82A	TCG TTG TCC GGG ATC CTG TAG CCA C	9432 to 9458
Exon IX	91	TCC GGG ATC CCG GCG CTC TAA CGG CG	9397 to 9422
	92A	GGT CAG GGA ATT CGG CTG CTC CCG CTT	9778 to 9804
Exon X	101	TAG GAA TTC GGG GGG GAA GGA GGA GCC GA	9664 to 9692
	102A	GTG GAA AGA AGC TTG GGG CGG GAG AAG GTA	10088 to 10117
Exon XI	111	AGG AAG CTT GAA CAC GGG ATT GGG GTT	10139 to 10165
	112A	TCC GGA TCCTCC TGA AGG CGC AAC AG	10462 to 10487
Exon XII	121	CCC GTA AGC TTC CAG CAC GAC CTG GGT	10354 to 10380
	122A	GGA ACG ATA CCG AAT TCG CGG GCT TCT T	10665 to 10692
Exon XIII	131	GAG AAG CTT TGT CCA TCG TCC GGG CGG C	11086 to 11113
	132A	TGT GGA CCT GAG AAT TCT GCC TGA CGG CCT	11341 to 11370
Exon XIV	141	GTG CCA GGT AAG CTT TTA GCC CGG TTG	11301 to 11327
	142A	ACA CAA TCT TGA ATT CCA TGC CCC AGC CA	11615 to 11643

“A” in the primer number denotes an antisense strand oligonucleotide. Nucleotide sequences designed for the digestion by restriction enzymes are underlined.

Nucleotide numberings are based on Cool and MacGillivray.6

†

Primer used for mutagenic PCR.

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