Table 2. Donor T-Cell Expansion and... | American Society of Hematology

Table 2.

Donor T-Cell Expansion and Engraftment After Transplantation

Donors	Day-7 Splenocytes		Day-60 PBC % H2-K^b+/H-2D^d−
Donors	Cells (×10⁶/spleen)	% T Cells	Day-60 PBC % H2-K^b+/H-2D^d−
B6	1.48	75.0	ND
GCSF-B6	1.75	60.2	99.0
pfp^−/−	1.90	69.6	ND
GCSF-pfp^−/−	2.05	64.4	99.0
B6D2F1	2.60	13.6	0.5

Donors	Day-7 Splenocytes		Day-60 PBC % H2-K^b+/H-2D^d−
Donors	Cells (×10⁶/spleen)	% T Cells	Day-60 PBC % H2-K^b+/H-2D^d−
B6	1.48	75.0	ND
GCSF-B6	1.75	60.2	99.0
pfp^−/−	1.90	69.6	ND
GCSF-pfp^−/−	2.05	64.4	99.0
B6D2F1	2.60	13.6	0.5

Total body irradiated B6D2F1 recipients received 1 × 10⁷ splenocytes from control B6 donors, G-CSF–mobilized B6 donors, pfp^−/− donors, G-CSF–mobilized pfp^−/− donors, or control B6D2F1 donors. On day-7 posttransplant, splenocytes were harvested (n = 5/group) and were counted using a hemocytometer. The percentage of T cells was determined by staining with FITC-conjugated anti-CD4 and PE-conjugated anti-CD8 Abs. On day 60 posttransplant, peripheral blood (N = 4) was collected and stained with FITC-conjugated anti–H-2K^b and PE-conjugated anti–H-2D^d.

Abbreviation: ND, not determined.

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