Cytokine Profile After G-CSF Mobilization
| Cytokines . | 1°MLR . | 2°MLR . | ||
|---|---|---|---|---|
| Control . | G-CSF . | Control . | G-CSF . | |
| IL-12 (pg/mL) | 16.9 ± 0.20 | 10.4 ± 0.23* | 1.7 ± 0.15 | <1.0* |
| IL-2 (U/mL) | 0.9 ± 0.03 | 0.7 ± 0.02* | <0.1 | <0.1 |
| IFN-γ (U/mL) | 19.1 ± 0.78 | 11.5 ± 1.25* | 485.7 ± 5.73 | 359.2 ± 17.12* |
| IL-4 (U/mL) | 0.4 ± 0.02 | 5.4 ± 0.14* | 6.88 ± 1.93 | 33.3 ± 1.84* |
| IL-10 (pg/mL) | 35.7 ± 2.28 | 50.2 ± 2.01* | 343.3 ± 46.04 | 616.8 ± 11.95* |
| Cytokines . | 1°MLR . | 2°MLR . | ||
|---|---|---|---|---|
| Control . | G-CSF . | Control . | G-CSF . | |
| IL-12 (pg/mL) | 16.9 ± 0.20 | 10.4 ± 0.23* | 1.7 ± 0.15 | <1.0* |
| IL-2 (U/mL) | 0.9 ± 0.03 | 0.7 ± 0.02* | <0.1 | <0.1 |
| IFN-γ (U/mL) | 19.1 ± 0.78 | 11.5 ± 1.25* | 485.7 ± 5.73 | 359.2 ± 17.12* |
| IL-4 (U/mL) | 0.4 ± 0.02 | 5.4 ± 0.14* | 6.88 ± 1.93 | 33.3 ± 1.84* |
| IL-10 (pg/mL) | 35.7 ± 2.28 | 50.2 ± 2.01* | 343.3 ± 46.04 | 616.8 ± 11.95* |
B6 donor mice were injected with human G-CSF (100 μg/kg/d) for 6 days, splenocytes were harvested on day 7, and T cells were enriched by passing through nylon-wool column. Results represent the mean ± SE from 6 to 9 samples/group.
Abbreviations: 1°MLR, splenic T cells from B6 donors were incubated with irradiated peritoneal cells from B6D2F1 mice for 48 hours, and cytokine levels in the culture supernatants were determined by ELISA; 2°MLR, cells from day-6 1°MLR were restimulated with irradiated peritoneal cells from B6D2F1 mice for 48 hours, and cytokine levels in the culture supernatants were determined by ELISA.
P < .01 v control B6 donors.