Phycoerythrin-Conjugated rhG-CSF (PE-G-CSF) Binding to LGM-1 Cells Transfected With Full-Length or Mutant G-CSFR Expression Plasmids
| Cell Line* . | Relative Fluorescence Intensity† . | ||
|---|---|---|---|
| Control . | PE-G-CSF . | PE-G-CSF + rhG-CSF . | |
| LGM V | 1.00 | 1.00 | 1.00 |
| LGM GR95 | 1.00 | 2.94 | 0.99 |
| LGM GR-A | 1.00 | 4.32 | 0.97 |
| LGM GR-B | 1.00 | 3.73 | 0.99 |
| LGM GR-C | 1.00 | 6.52 | 1.00 |
| LGM GR-D | 1.00 | 6.10 | 1.04 |
| LGM GR-E | 1.00 | 4.06 | 1.01 |
| LGM GR-F | 1.00 | 2.44 | 0.98 |
| Cell Line* . | Relative Fluorescence Intensity† . | ||
|---|---|---|---|
| Control . | PE-G-CSF . | PE-G-CSF + rhG-CSF . | |
| LGM V | 1.00 | 1.00 | 1.00 |
| LGM GR95 | 1.00 | 2.94 | 0.99 |
| LGM GR-A | 1.00 | 4.32 | 0.97 |
| LGM GR-B | 1.00 | 3.73 | 0.99 |
| LGM GR-C | 1.00 | 6.52 | 1.00 |
| LGM GR-D | 1.00 | 6.10 | 1.04 |
| LGM GR-E | 1.00 | 4.06 | 1.01 |
| LGM GR-F | 1.00 | 2.44 | 0.98 |
LGM-1 cells were transfected with vector alone, the full-length G-CSFR expression plasmid, or mutant G-CSFR expression plasmids to generate LGM V, LGM GR95, LGM GR-A, LGM GR-B, LGM GR-C, LGM GR-D, LGM GR-E, and LGM GR-F cells, respectively.
Transfected cells were stained with PE-G-CSF with or without preincubation in a 10-fold molar excess of unconjugated cytokine. Controls are transfected cells stained with phycoerythrin-conjugated streptavidin. The fluorescence intensity of controls was defined as 1.00, and the numbers indicate the representative fold change in fluorescence intensity compared with controls.