Expression of Cell-Surface HS Proteoglycans
| MoAb Against . | HEL Supernatant/ Extract . | ELM-D Supernatant/ Extract . | Cervix Fibroblasts Cell Extract . |
|---|---|---|---|
| Syndecan-2 (6G12) | −/− | −/− | + |
| Syndecan-4 (8G3) | +/+ | +/(+) | + |
| Glypican (S1) | −/− | +/(+) | + |
| HS proteoglycans (3G10) | +/+ | +/+ | + |
| MoAb Against . | HEL Supernatant/ Extract . | ELM-D Supernatant/ Extract . | Cervix Fibroblasts Cell Extract . |
|---|---|---|---|
| Syndecan-2 (6G12) | −/− | −/− | + |
| Syndecan-4 (8G3) | +/+ | +/(+) | + |
| Glypican (S1) | −/− | +/(+) | + |
| HS proteoglycans (3G10) | +/+ | +/+ | + |
5 μL of proteoglycan-containing samples were applied to Biodyne B membrane; subsequently, membranes were blocked by 5% BSA/5% dry milk as described in Materials and Methods. Detection was performed by the use of the indicated specific MoAbs against the different HS proteoglycans and the ECL-system. Very weak staining for syndecan-4 and glypican in the cell extract is indicated by (+).