Levels of icRHAMM, icCD44, and icHA Binding Are Significantly Greater in Steady-State BM HPCs Than in Mobilized Blood HPCs, and Levels of icRHAMM Are Significantly Greater in Steady-State BM HPCs as Compared With Mobilized BM HPCs
| . | MFI Treatment of Cells Before Staining . | Ratio of Staining . | |
|---|---|---|---|
| Untreated . | Permeabilized . | Intact:Permeabilized . | |
| Steady-state BM HPCs (11) | |||
| RHAMM | 14 ± 2 | 311 ± 463-150 | 21.3 ± 1.83-150,3-151 |
| CD44 | 74 ± 8 | 416 ± 50 | 5.5 ± 0.73-152 |
| HA binding | 7 ± 1 | 125 ± 21 | 22.7 ± 4.83-153,3-151 |
| Mobilized BM HPCs (4) | |||
| RHAMM | 15 ± 2 | 141 ± 323-155 | 9.3 ± 1.93-154 |
| CD44 | 81 ± 8 | 251 ± 66 | 3.9 ± 1.1 |
| HA binding | 14 ± 6 | 226 ± 112 | 13.7 ± 3.1 |
| . | MFI Treatment of Cells Before Staining . | Ratio of Staining . | |
|---|---|---|---|
| Untreated . | Permeabilized . | Intact:Permeabilized . | |
| Steady-state BM HPCs (11) | |||
| RHAMM | 14 ± 2 | 311 ± 463-150 | 21.3 ± 1.83-150,3-151 |
| CD44 | 74 ± 8 | 416 ± 50 | 5.5 ± 0.73-152 |
| HA binding | 7 ± 1 | 125 ± 21 | 22.7 ± 4.83-153,3-151 |
| Mobilized BM HPCs (4) | |||
| RHAMM | 15 ± 2 | 141 ± 323-155 | 9.3 ± 1.93-154 |
| CD44 | 81 ± 8 | 251 ± 66 | 3.9 ± 1.1 |
| HA binding | 14 ± 6 | 226 ± 112 | 13.7 ± 3.1 |
Values are the mean ± SE. The number in brackets is the number of individual patient samples. Staining, permeabilization, and analysis was as for Table 2. Mobilized BM HPC values were not significantly different from those of paired mobilized blood HPC samples.
P < .001 as compared with the values obtained for mobilized blood HPCs as shown in Table 2.
P = .004 as compared with ratios for CD44 in SS-BM HPCs.
P < .004 as compared with the values obtained for mobilized blood HPCs as shown in Table 2.
P < .01 as compared with the values obtained for mobilized blood HPCs as shown in Table 2.
P = .04 as compared with steady-state BM HPCs.
P = .002 as compared with steady-state BM.