Mobilized Blood HPCs Have High Levels of Cytoplasmic HA Binding and of RHAMM, But Not of CD44
| . | MFI Treatment of HPCs Before Staining . | Ratio of Staining . | |
|---|---|---|---|
| Untreated . | Permeabilized . | Untreated:Permeabilized . | |
| RHAMM | 14 ± 2 | 121 ± 17 | 12.5 ± 1.7* |
| CD44 | 85 ± 6 | 212 ± 28 | 2.4 ± 0.3 |
| HA binding | 14 ± 2 | 107 ± 64 | 9.3 ± 2* |
| . | MFI Treatment of HPCs Before Staining . | Ratio of Staining . | |
|---|---|---|---|
| Untreated . | Permeabilized . | Untreated:Permeabilized . | |
| RHAMM | 14 ± 2 | 121 ± 17 | 12.5 ± 1.7* |
| CD44 | 85 ± 6 | 212 ± 28 | 2.4 ± 0.3 |
| HA binding | 14 ± 2 | 107 ± 64 | 9.3 ± 2* |
Cells were treated with phosphate-buffered saline or permeabilized followed by staining in three-color IF with CD34-PE, CD45-QR, and HA-FITC or MoAb to RHAMM-FITC (3T3.5), CD44-FITC (50B4), or isotype-matched controls. Files were gated for HPCs, and the staining for HA-binding or MoAb to RHAMM or CD44 was plotted as a histogram and compared with that of the isotype controls. Values are the mean ± SE of 24 samples. Ratios were calculated individually for each sample before calculating the mean values. MFI is the mean channel of staining for those cells with an intensity above that of identically gated isotype-matched control MoAbs. Statistics were as for Table 1.
P < .001 as compared with the ratio for CD44.