Cell Surface Expression of RHAMM, CD44, and HA Binding by CD34+45lo HPCs From Mobilized Blood and Steady-State BM
| . | Source of HPCs . | |||
|---|---|---|---|---|
| Mobilized Blood . | Steady-State BM . | |||
| % of HPC . | MFI . | % of HPC . | MFI . | |
| RHAMM | 43 ± 6* | 14 ± 2 | 76 ± 6 | 14 ± 2 |
| CD44 | 74 ± 6* | 85 ± 6 | 100 ± 0 | 75 ± 9 |
| HA binding | 67 ± 7† | 14 ± 2 | 70 ± 13 | 7 ± 1 |
| . | Source of HPCs . | |||
|---|---|---|---|---|
| Mobilized Blood . | Steady-State BM . | |||
| % of HPC . | MFI . | % of HPC . | MFI . | |
| RHAMM | 43 ± 6* | 14 ± 2 | 76 ± 6 | 14 ± 2 |
| CD44 | 74 ± 6* | 85 ± 6 | 100 ± 0 | 75 ± 9 |
| HA binding | 67 ± 7† | 14 ± 2 | 70 ± 13 | 7 ± 1 |
Values are the mean ± SE for HPCs from 33 mobilized blood samples and 10 SS-BM samples. Staining was by three-color IF using CD34-PE, CD45-PerCP, and either IgG1-FITC, RHAMM-FITC, CD44-FITC, or HA-FITC in comparison to identically gated isotype-matched controls.
P ≤ .008 as compared with steady-state BM HPCs using the Student’s t-test for samples with a normal distribution and the Mann Whitney rank sum test for samples failing normality or equal variance.
Not significant.