Antibody Staining of PU.1−/− Myeloid Colonies
| Markers . | Percent Positive* . |
|---|---|
| ER-MP12 | >90% |
| ER-MP58 | >90% |
| ER-MP20 | 30%-50% |
| c-Kit | >90% |
| Gr-1 | 10%-20% |
| Mac-1 | <1% |
| F4/80 | 0 |
| Markers . | Percent Positive* . |
|---|---|
| ER-MP12 | >90% |
| ER-MP58 | >90% |
| ER-MP20 | 30%-50% |
| c-Kit | >90% |
| Gr-1 | 10%-20% |
| Mac-1 | <1% |
| F4/80 | 0 |
Seven-day PU.1−/− myeloid colonies were pooled and cytospun onto glass slides. Cells were fixed and stained as indicated in Materials and Methods. No background staining was observed with isotype antibody controls.
The percentage of cells positive for the antibody tested is given.