T-Cell Differentiation of Precursors Originating From Different Embryonic Tissues
| Tissue Analyzed . | Days of Development . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| E4 . | E7 . | E10 . | E11 . | E12 . | E13 . | E14 . | E15 . | E16 . | E19 . | E23 . | |
| Paraaortic foci | |||||||||||
| 1,2 embryo | 4/4 (8.4) | 3/3 (2.3) | |||||||||
| 0,12 embryo | 2/4 (0.3) | ||||||||||
| Blood | |||||||||||
| 24 × 106 cells | 1/1 (7.0) | 1/1 (8.0) | 1/1 (10.0) | ||||||||
| 12 × 106 cells | 5/6 (1.7) | 10/12 (4.3) | 6/10 (3.4) | 8/9 (10.0) | 4/4 (4.5) | 5/6 (2.0) | |||||
| 3 × 106 cells | 3/6 (0.4) | 0/6 (0.0) | 4/6 (1.2) | 0/6 (0.0) | 3/3 (5.3) | 3/3 (4.5) | |||||
| 3 × 105 cells | 0/3 (0.0) | 0/3 (0.0) | 0/3 (0.0) | 0/3 (0.0) | 0/2 (0.0) | 0/2 (0.0) | |||||
| Spleen | |||||||||||
| 3 × 105 cells | 2/4 (0.2) | 4/4 (7.0) | 4/4 (65.8) | 3/3 (47.7) | 3/3 (30.9) | 4/4 (64.0) | |||||
| 3 × 104 cells | 0/2 (0.0) | 3/3 (1.1) | 3/3 (17.6) | 3/3 (20.0) | 3/3 (11.0) | 2/2 (31.4) | |||||
| BM | |||||||||||
| 3 × 105cells | 3/3 (4.3) | 5/5 (65.4) | 3/3 (36.0) | 3/3 (43.4) | 2/2 (55.1) | ||||||
| 3 × 104 cells | 3/3 (0.4) | 3/3 (23.8) | 3/3 (17.3) | 3/3 (17.0) | 1/1 (35.0) | ||||||
| Tissue Analyzed . | Days of Development . | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| E4 . | E7 . | E10 . | E11 . | E12 . | E13 . | E14 . | E15 . | E16 . | E19 . | E23 . | |
| Paraaortic foci | |||||||||||
| 1,2 embryo | 4/4 (8.4) | 3/3 (2.3) | |||||||||
| 0,12 embryo | 2/4 (0.3) | ||||||||||
| Blood | |||||||||||
| 24 × 106 cells | 1/1 (7.0) | 1/1 (8.0) | 1/1 (10.0) | ||||||||
| 12 × 106 cells | 5/6 (1.7) | 10/12 (4.3) | 6/10 (3.4) | 8/9 (10.0) | 4/4 (4.5) | 5/6 (2.0) | |||||
| 3 × 106 cells | 3/6 (0.4) | 0/6 (0.0) | 4/6 (1.2) | 0/6 (0.0) | 3/3 (5.3) | 3/3 (4.5) | |||||
| 3 × 105 cells | 0/3 (0.0) | 0/3 (0.0) | 0/3 (0.0) | 0/3 (0.0) | 0/2 (0.0) | 0/2 (0.0) | |||||
| Spleen | |||||||||||
| 3 × 105 cells | 2/4 (0.2) | 4/4 (7.0) | 4/4 (65.8) | 3/3 (47.7) | 3/3 (30.9) | 4/4 (64.0) | |||||
| 3 × 104 cells | 0/2 (0.0) | 3/3 (1.1) | 3/3 (17.6) | 3/3 (20.0) | 3/3 (11.0) | 2/2 (31.4) | |||||
| BM | |||||||||||
| 3 × 105cells | 3/3 (4.3) | 5/5 (65.4) | 3/3 (36.0) | 3/3 (43.4) | 2/2 (55.1) | ||||||
| 3 × 104 cells | 3/3 (0.4) | 3/3 (23.8) | 3/3 (17.3) | 3/3 (17.0) | 1/1 (35.0) | ||||||
T-cell differentiation was analyzed after intrathymic injection of cells into sublethally irradiated 10-day-old congenic chick and in vivo differentiation for 14 days. For tissues older than day 16, injection was performed with TCR− cells to eliminate eventual artefactual proliferation of mature T cells in our assay. No differences were observed between TCR− and total cell injection, confirming our negative control obtained with TCR+ T-cell injection. Data are expressed in number of recipients containing donor thymocytes per total number of injected. Numbers in parentheses correspond to the mean of the percentage of donor thymocyte, negative recipients were taken into account in this calculation (0%). For convenience, E23 corresponds to 2-day-old chicks.