Table 2.

Megakaryocyte Colony Formation From Cord Blood CD34+ Cells in Serum-Free Methylcellulose Culture

Factors Number of Colonies/500 Cells
CFU-Mk BFU-Mk Mk-mix Total
No factors  0 0  0  0  
IL-6 + SCF  1 ± 1.5  0 0  1 ± 1.5  
sIL-6R + IL-6 + SCF 9.7 ± 4.2* 3.3 ± 1.5  22.7 ± 3.5 35.7 ± 6.5* 
IL-3  2.7 ± 1.2  0 0  2.7 ± 1.2  
IL-6 + IL-3  3.7 ± 0.6 0.3 ± 0.6  2.0 ± 1.0  6.0 ± 1.0 
sIL-6R + IL-6 + IL-3  4.7 ± 1.2  1.7 ± 1.6* 4.3 ± 1.5  10.7 ± 2.9* 
TPO  24.0 ± 3.6 1.3 ± 1.5  0  25.3 ± 3.1  
IL-6 + TPO 26.0 ± 5.3  2.0 ± 1.5  0  27.1 ± 2.6 
sIL-6R + IL-6 + TPO  27.3 ± 2.1  6.0 ± 1.6* 0  33.3 ± 2.8* 
SCF + TPO  32.0 ± 7.0 17.7 ± 6.4  1.6 ± 1.5  51.3 ± 13.7 
IL-6 + SCF + TPO  29.5 ± 8.0  20.7 ± 4.8 2.1 ± 1.0  52.0 ± 9.8 
sIL-6R + IL-6 + SCF  + TPO  31.0 ± 2.6 10.3 ± 2.3  53.0 ± 3.0* 94.0 ± 4.0* 
SCF + IL-3 + TPO  30.6 ± 5.5  18.3 ± 1.5 4.0 ± 1.0  53.0 ± 6.1 
SCF + IL-3 + TPO  + IL-6  32.0 ± 3.0 17.0 ± 2.6  7.3 ± 2.1  56.3 ± 3.1 
Factors Number of Colonies/500 Cells
CFU-Mk BFU-Mk Mk-mix Total
No factors  0 0  0  0  
IL-6 + SCF  1 ± 1.5  0 0  1 ± 1.5  
sIL-6R + IL-6 + SCF 9.7 ± 4.2* 3.3 ± 1.5  22.7 ± 3.5 35.7 ± 6.5* 
IL-3  2.7 ± 1.2  0 0  2.7 ± 1.2  
IL-6 + IL-3  3.7 ± 0.6 0.3 ± 0.6  2.0 ± 1.0  6.0 ± 1.0 
sIL-6R + IL-6 + IL-3  4.7 ± 1.2  1.7 ± 1.6* 4.3 ± 1.5  10.7 ± 2.9* 
TPO  24.0 ± 3.6 1.3 ± 1.5  0  25.3 ± 3.1  
IL-6 + TPO 26.0 ± 5.3  2.0 ± 1.5  0  27.1 ± 2.6 
sIL-6R + IL-6 + TPO  27.3 ± 2.1  6.0 ± 1.6* 0  33.3 ± 2.8* 
SCF + TPO  32.0 ± 7.0 17.7 ± 6.4  1.6 ± 1.5  51.3 ± 13.7 
IL-6 + SCF + TPO  29.5 ± 8.0  20.7 ± 4.8 2.1 ± 1.0  52.0 ± 9.8 
sIL-6R + IL-6 + SCF  + TPO  31.0 ± 2.6 10.3 ± 2.3  53.0 ± 3.0* 94.0 ± 4.0* 
SCF + IL-3 + TPO  30.6 ± 5.5  18.3 ± 1.5 4.0 ± 1.0  53.0 ± 6.1 
SCF + IL-3 + TPO  + IL-6  32.0 ± 3.0 17.0 ± 2.6  7.3 ± 2.1  56.3 ± 3.1 

Five hundred cord blood CD34+ cells/mL were initiated and Mk colonies were scored at day 11. Results are represented by mean ± SD from triplicate cultures.

*

Significantly different from their counterparts without sIL-6R (P < .05 to .0001).

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