DNA Fragments Used in the Constructs
| Fragment . | GenBank humhbb Coordinate . | Content of Fragment . |
|---|---|---|
| StuI/HindIII | 39050-41382 | Aγ-globin gene with −382 promoter |
| ApaI/HindIII | 39230-41382 | Aγ-globin gene with −201 promoter |
| AvaII/HindIII | 39271-41382 | Aγ-globin gene with −159 promoter |
| NcoI/HindIII | 39290-41382 | Aγ-globin gene with −141 promoter |
| HindIII/HindIII | 41382-42135 | Aγ-globin gene 3′ enhancer |
| Bgl II/Bgl II | 60629-65610 | β-globin gene with −1560 promoter |
| HpaI/Bgl II | 61372-65610 | β-globin gene with −614 promoter |
| SnaBI/Bgl II | 61921-65610 | β-globin gene with −267 promoter |
| RsaI/Bgl II | 62060-65610 | β-globin gene with −127 promoter |
| AvrII/Bgl II | 62101-65610 | β-globin gene with −86 promoter |
| BspHI/Bgl II | 64081-65610 | β-globin gene 3′ enhancer |
| PstI/PstI | 4348-5132 | HS3 |
| HindIII/AluI | 8486-8784 | HS2 |
| TaqI/XmnI | αHS-40 |
| Fragment . | GenBank humhbb Coordinate . | Content of Fragment . |
|---|---|---|
| StuI/HindIII | 39050-41382 | Aγ-globin gene with −382 promoter |
| ApaI/HindIII | 39230-41382 | Aγ-globin gene with −201 promoter |
| AvaII/HindIII | 39271-41382 | Aγ-globin gene with −159 promoter |
| NcoI/HindIII | 39290-41382 | Aγ-globin gene with −141 promoter |
| HindIII/HindIII | 41382-42135 | Aγ-globin gene 3′ enhancer |
| Bgl II/Bgl II | 60629-65610 | β-globin gene with −1560 promoter |
| HpaI/Bgl II | 61372-65610 | β-globin gene with −614 promoter |
| SnaBI/Bgl II | 61921-65610 | β-globin gene with −267 promoter |
| RsaI/Bgl II | 62060-65610 | β-globin gene with −127 promoter |
| AvrII/Bgl II | 62101-65610 | β-globin gene with −86 promoter |
| BspHI/Bgl II | 64081-65610 | β-globin gene 3′ enhancer |
| PstI/PstI | 4348-5132 | HS3 |
| HindIII/AluI | 8486-8784 | HS2 |
| TaqI/XmnI | αHS-40 |