IL-6 and EPO Instead of IL-3 Do Not Preserve SRC Numbers in Culture
| Days in Culture/ Cytokines in Culture . | % CD45+Cells4-150 . | SRC Frequency . | 95% CI . |
|---|---|---|---|
| Day 0 | 12.5 ± 8.4 | 1/17,100 | 9,100-31,900 |
| Day 1 | |||
| SF, FL, IL-3 | 5.2 ± 6.4 | 1/38,800 | 22,100-68,100 |
| SF, FL, IL-6, EPO | 1.1 ± 1.1 | 1/39,100 | 17,500-87,700 |
| Day 3 | |||
| SF, FL, IL-3 | 0.5 ± 0.5 | 1/70,700 | 44,400-112,500 |
| SF, FL, IL-6, EPO | 3.3 ± 4.5 | 1/87,700 | 37,500-205,200 |
| Days in Culture/ Cytokines in Culture . | % CD45+Cells4-150 . | SRC Frequency . | 95% CI . |
|---|---|---|---|
| Day 0 | 12.5 ± 8.4 | 1/17,100 | 9,100-31,900 |
| Day 1 | |||
| SF, FL, IL-3 | 5.2 ± 6.4 | 1/38,800 | 22,100-68,100 |
| SF, FL, IL-6, EPO | 1.1 ± 1.1 | 1/39,100 | 17,500-87,700 |
| Day 3 | |||
| SF, FL, IL-3 | 0.5 ± 0.5 | 1/70,700 | 44,400-112,500 |
| SF, FL, IL-6, EPO | 3.3 ± 4.5 | 1/87,700 | 37,500-205,200 |
Mean ± SD for human cells found in the BM of engrafted mice after injection of 105 CB cells; 10-15 mice were analyzed per point. Day 0 v day 1 (both cytokine combinations), not significant; day 0 v day 3 (SF, FL, IL-3), P < .001; day 0 v day 3 (SF, FL, IL-6, EPO), P < .005.