Location and Length of ERGIC-53 Exons and Sequences of Primers Used to PCR Complete Coding Region of ERGIC-53 From Genomic DNA
| Exon . | Nucleotides* . | Exon Length . | Primers . |
|---|---|---|---|
| 1 | ?-235 | 235+ | 5′-TCGCGTTCCAGAATCCAAG-3′ |
| 5′-TCAGCACACCAGGGTAGC-3′ | |||
| 2 | 236-390 | 155 | 5′-CAGTTTGGAAATGTACATTGAG-3′ |
| 5′-GGGAACAGTTAGAGGCTAG-3′ | |||
| 3 | 391-498 | 108 | 5′-CATGCCTCTAACTGTTCCC-3′ |
| 5′-CTCACAGCCTAACTCTGTTG-3′ | |||
| 4 | 499-560 | 62 | 5′-TGTAAGTCACTTCATAGTAC-3′ |
| 5′-CAATGTATTTCATAAGGATTCC-3′ | |||
| 5 | 561-660 | 100 | 5′-TGAAAAGCTGAGTGTCTTGT-3′ |
| 5′-GAAAAGTGATACTGTAACATTG-3′ | |||
| 6 | 661-784 | 124 | 5′-GAAACAAAACTGAATAGTAGTC-3′ |
| 5′-ACAAGTCTACATATCCCTAAv | |||
| 7 | 785-843 | 59 | 5′-AGAGTGCCATTGCCTTTACC-3′ |
| 5′-CAAACCTAAGTTAGTCTTCC-3′ | |||
| 8 | 844-976 | 132 | 5′-CATGTATAGAGATATCTTAATG-3′ |
| 5′-GTCCATGATCAACAGCCTC-3′ | |||
| 9 | 977-1170 | 194 | 5′-CACTTTGGTCACTTACGTTA-3′ |
| 5′-TCTATGAGCACATAGTACAG-3′ | |||
| 10 | 1171-1241 | 71 | 5′-GGGAAGTAAAGAAGAAGGGC-3′ |
| 5′-AATCACATAACACACAAACG-3′ | |||
| 11 | 1242-1395 | 154 | 5′-GTGATTTTATTGTATCAAGAG-3′ |
| 5′-AGTATGAGTTCTTCCTTTCC-3′ | |||
| 12 | 1396-1517 | 122 | 5′-GGGGATAGAAGGTTTTCTGG-3′ |
| 5′-GAACATAGATAACTTAGTTG-3′ | |||
| 13 | 1518-? | 1244+ | 5′-CTGTTCATTTCAGTTCACAT-3′ |
| 5′-AATTCCCTCAAAACGACATC-3′ |
| Exon . | Nucleotides* . | Exon Length . | Primers . |
|---|---|---|---|
| 1 | ?-235 | 235+ | 5′-TCGCGTTCCAGAATCCAAG-3′ |
| 5′-TCAGCACACCAGGGTAGC-3′ | |||
| 2 | 236-390 | 155 | 5′-CAGTTTGGAAATGTACATTGAG-3′ |
| 5′-GGGAACAGTTAGAGGCTAG-3′ | |||
| 3 | 391-498 | 108 | 5′-CATGCCTCTAACTGTTCCC-3′ |
| 5′-CTCACAGCCTAACTCTGTTG-3′ | |||
| 4 | 499-560 | 62 | 5′-TGTAAGTCACTTCATAGTAC-3′ |
| 5′-CAATGTATTTCATAAGGATTCC-3′ | |||
| 5 | 561-660 | 100 | 5′-TGAAAAGCTGAGTGTCTTGT-3′ |
| 5′-GAAAAGTGATACTGTAACATTG-3′ | |||
| 6 | 661-784 | 124 | 5′-GAAACAAAACTGAATAGTAGTC-3′ |
| 5′-ACAAGTCTACATATCCCTAAv | |||
| 7 | 785-843 | 59 | 5′-AGAGTGCCATTGCCTTTACC-3′ |
| 5′-CAAACCTAAGTTAGTCTTCC-3′ | |||
| 8 | 844-976 | 132 | 5′-CATGTATAGAGATATCTTAATG-3′ |
| 5′-GTCCATGATCAACAGCCTC-3′ | |||
| 9 | 977-1170 | 194 | 5′-CACTTTGGTCACTTACGTTA-3′ |
| 5′-TCTATGAGCACATAGTACAG-3′ | |||
| 10 | 1171-1241 | 71 | 5′-GGGAAGTAAAGAAGAAGGGC-3′ |
| 5′-AATCACATAACACACAAACG-3′ | |||
| 11 | 1242-1395 | 154 | 5′-GTGATTTTATTGTATCAAGAG-3′ |
| 5′-AGTATGAGTTCTTCCTTTCC-3′ | |||
| 12 | 1396-1517 | 122 | 5′-GGGGATAGAAGGTTTTCTGG-3′ |
| 5′-GAACATAGATAACTTAGTTG-3′ | |||
| 13 | 1518-? | 1244+ | 5′-CTGTTCATTTCAGTTCACAT-3′ |
| 5′-AATTCCCTCAAAACGACATC-3′ |