Incidence of Hematopoietic and Endothelial Cell Differentiation From TEK+ Cells Derived From AGM Cells at 10.5 dpc
| Variable . | No. of Wells . | |||
|---|---|---|---|---|
| IL-3 . | IL-3 + Angiopoietin-1 . | |||
| Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | |
| Seeded cells | 600 | 1,152 | 400 | 411 |
| Growth of hematopoietic cells | 6 | 13 | 5 | 5 |
| Growth of hematopoietic and endothelial cells | 0 | 0 | 3 | 3 |
| Variable . | No. of Wells . | |||
|---|---|---|---|---|
| IL-3 . | IL-3 + Angiopoietin-1 . | |||
| Exp 1 . | Exp 2 . | Exp 1 . | Exp 2 . | |
| Seeded cells | 600 | 1,152 | 400 | 411 |
| Growth of hematopoietic cells | 6 | 13 | 5 | 5 |
| Growth of hematopoietic and endothelial cells | 0 | 0 | 3 | 3 |
Single TEK+ cells were cocultured with OP9 cells in the presence of IL-3 (100 U/mL) with or without angiopoietin-1 (100 ng/mL). Hematopoietic cells were developed on OP9 cells after 7 days’ culture. These OP9 cell layers were immunostained with PECAM-1 MoAb.