Incidence of Colony-Forming Cells: Stem-Cell Fraction of Cultured AGM Cells
| Lin−,c-Kit+,Sca-1+Cells . | No. of Colonies (mean ± SD) . | |||||
|---|---|---|---|---|---|---|
| Origin . | No./Dish . | GM . | M . | Meg . | E/MIX . | Total . |
| AGM (cultured) | 300 | 27.5 ± 4.7 | 3.3 ± 1.0 | 0.5 ± 1.0 | 0.3 ± 0.5 | 31.5 ± 5.8 |
| Bone marrow | 300 | 114.8 ± 8.3 | 19.0 ± 3.2 | 6.3 ± 3.0 | 2.0 ± 1.8 | 142.0 ± 8.6 |
| Lin−,c-Kit+,Sca-1+Cells . | No. of Colonies (mean ± SD) . | |||||
|---|---|---|---|---|---|---|
| Origin . | No./Dish . | GM . | M . | Meg . | E/MIX . | Total . |
| AGM (cultured) | 300 | 27.5 ± 4.7 | 3.3 ± 1.0 | 0.5 ± 1.0 | 0.3 ± 0.5 | 31.5 ± 5.8 |
| Bone marrow | 300 | 114.8 ± 8.3 | 19.0 ± 3.2 | 6.3 ± 3.0 | 2.0 ± 1.8 | 142.0 ± 8.6 |
For the colony assay, 300 sorted cells were embedded in 1 mL of methylcellulose medium containing SCF (100 ng/mL), IL-3 (200 U/mL), IL-6 (20 ng/mL), and Epo (2 U/mL). The numbers of colonies were scored on day 7 to 10 of culture.