Incidence of Colony-Forming Cells: Primary AGM Cells
| Origin . | No. of Colonies*/1,000 Cells (mean ± SD) . | ||||
|---|---|---|---|---|---|
| G/GM . | M . | Meg . | E/MIX . | Total . | |
| Total AGM cells | 5.8 ± 3.2 | 3.5 ± 1.2 | 1.0 ± 1.0 | 4.5 ± 1.3 | 14.8 ± 2.6 |
| AGM TEK+ cells | 12.0 ± 1.0 | 9.0 ± 2.6 | 2.0 ± 1.0 | 6.3 ± 0.6 | 29.3 ± 2.3 |
| AGM TEK− cells | 0 | 0 | 0 | 0 | 0 |
| Bone marrow TEK+cells | 18.3 ± 4.0 | 4.5 ± 2.5 | 0.5 ± 0.6 | 0 | 23.3 ± 5.1 |
| Origin . | No. of Colonies*/1,000 Cells (mean ± SD) . | ||||
|---|---|---|---|---|---|
| G/GM . | M . | Meg . | E/MIX . | Total . | |
| Total AGM cells | 5.8 ± 3.2 | 3.5 ± 1.2 | 1.0 ± 1.0 | 4.5 ± 1.3 | 14.8 ± 2.6 |
| AGM TEK+ cells | 12.0 ± 1.0 | 9.0 ± 2.6 | 2.0 ± 1.0 | 6.3 ± 0.6 | 29.3 ± 2.3 |
| AGM TEK− cells | 0 | 0 | 0 | 0 | 0 |
| Bone marrow TEK+cells | 18.3 ± 4.0 | 4.5 ± 2.5 | 0.5 ± 0.6 | 0 | 23.3 ± 5.1 |
For the colony assay, 1,000 sorted cells were embedded in 1 mL of methylcellulose medium containing SCF (100 ng/mL), IL-3 (200 U/mL), IL-6 (20 ng/mL), and Epo (2 U/mL). The numbers of colonies were scored on day 7 to 10 of culture.
Abbreviations: G/GM, granulocyte/granulocyte-macrophage colonies; M, macrophage colonies; Meg, megakaryocyte colonies; E/MIX, erythroid burst/megakaryocyte colonies.