Effect of Leptin on Apoptosis of MO7E and TF-1 Cells
| Medium . | Day 2 . | Day 3 . | |||
|---|---|---|---|---|---|
| FCS . | Leptin . | MO7E . | TF1 . | MO7E . | TF1 . |
| + | − | 6.4 | 2.9 | 44.8 | 47.9 |
| + | + | 4.5 | 2.0 | 11.2 | 17.5 |
| − | − | 60.3 | 7.5 | 78.1 | 68.9 |
| − | + | 27.7 | 3.5 | 54.6 | 31.8 |
| Medium . | Day 2 . | Day 3 . | |||
|---|---|---|---|---|---|
| FCS . | Leptin . | MO7E . | TF1 . | MO7E . | TF1 . |
| + | − | 6.4 | 2.9 | 44.8 | 47.9 |
| + | + | 4.5 | 2.0 | 11.2 | 17.5 |
| − | − | 60.3 | 7.5 | 78.1 | 68.9 |
| − | + | 27.7 | 3.5 | 54.6 | 31.8 |
Cytokine-deprived MO7E and TF-1 cells were cultured with or without fetal calf serum in the presence or absence of rH leptin. After 2 and 3 days, the cells were stained with acridine orange for cellular DNA and RNA content and analyzed on a FACScan flow cytometer. The percentage of cells in the subG1 region defined the proportion of apoptotic cells. Results represent the data of one of two independent experiments.