Table 2.

OB-R Expression in Hematopoietic Cell Lines

Cell Type LongOB-R Isoform Short OB-R Isoform
K562  ++ ++  
MO7E  ++  ++  
HL-60  +  +  
HL-60-DOX  +  
NB4  +  +  
KG1A  +  +  
OCI-AML 2  +  
OCI-AML 3  +  +  
BV173  +  +  
Jurkat  ++  
Raji  +  ++ 
Cell Type LongOB-R Isoform Short OB-R Isoform
K562  ++ ++  
MO7E  ++  ++  
HL-60  +  +  
HL-60-DOX  +  
NB4  +  +  
KG1A  +  +  
OCI-AML 2  +  
OCI-AML 3  +  +  
BV173  +  +  
Jurkat  ++  
Raji  +  ++ 

OB-R expression was determined by PCR using primers specific for the long and short isoforms. Estimates of OB-Rexpression were evaluated by visual comparison withβ2-microglobulin amplification.

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