aPTT Mixing Studies, Baseline, and Fourth Falloff Plasma Samples
| Animal . | Falloff 1 Baseline Plasma . | Falloff 4 Baseline Plasma . |
|---|---|---|
| 84269 | 29.3 seconds (2.9) | 26.3 seconds (2.0) |
| RQ1234 | 30.0 seconds (3.0) | 30.2 seconds (2.1) |
| RQ1305 | 32.4 seconds (3.5) | 31.3 seconds (3.3) |
| Animal . | Falloff 1 Baseline Plasma . | Falloff 4 Baseline Plasma . |
|---|---|---|
| 84269 | 29.3 seconds (2.9) | 26.3 seconds (2.0) |
| RQ1234 | 30.0 seconds (3.0) | 30.2 seconds (2.1) |
| RQ1305 | 32.4 seconds (3.5) | 31.3 seconds (3.3) |
aPTT’s were measured on 50:50 mix of heat-inactivated rhesus plasma and pooled normal human plasma using a factor-sensitive aPTT reagent. All values are mean of five determinations with standard deviation in parentheses. aPTT of normal pooled human plasma = 28.6 seconds (4.9), aPTT of 50:50 mix of normal pooled human plasma with Owren’s buffer = 27.3 seconds (1.5). A 1:5 dilution of a human factor IX inhibitor plasma with a Bethesda titer of ∼100 units was mixed 50:50 with normal pooled human plasma resulting in a mean aPTT of 62.3 seconds (average of two determinations, +/−3.7 seconds).