Effects of SDF-1 on Migration of CD41+Cells by Checkerboard Assay
| SDF-1α Concentration (ng/mL) in Lower Chamber . | SDF-1α Concentration (ng/mL) in Upper Chamber . | |||
|---|---|---|---|---|
| 0 . | 10 . | 50 . | 1,000 . | |
| 0 | 2.5 | 1.5 | 2 | 1.6 |
| 10 | 16 | 2.5 | 2 | 2.3 |
| 50 | 28.8 | 10.7 | 3.5 | 2.5 |
| 500 | 24.2 | 18.1 | 9.3 | 3 |
| 1,000 | 13 | 8.8 | 5.9 | 2.6 |
| SDF-1α Concentration (ng/mL) in Lower Chamber . | SDF-1α Concentration (ng/mL) in Upper Chamber . | |||
|---|---|---|---|---|
| 0 . | 10 . | 50 . | 1,000 . | |
| 0 | 2.5 | 1.5 | 2 | 1.6 |
| 10 | 16 | 2.5 | 2 | 2.3 |
| 50 | 28.8 | 10.7 | 3.5 | 2.5 |
| 500 | 24.2 | 18.1 | 9.3 | 3 |
| 1,000 | 13 | 8.8 | 5.9 | 2.6 |
Cells were added to the upper chamber and SDF-1α was added to either the lower or upper chamber. Results shown are the percentages of cells that migrated into the lower chamber and are representative of two independent experiments performed in duplicate.