Table 1. Recovery of PKH26 Cells From... | American Society of Hematology

Table 1.

Recovery of PKH26⁺ Cells From Primary Recipients 48 Hours Posttransplant

Source of PKH26⁺ Cells	Tissue Examined	% PKH26⁺ Cells Recovered^*
FR25Lin⁻	BM	12.75 ± 1.0
FR25Lin⁻	Spleen	0.27 ± 0.02
R/O	BM	1.25 ± 0.55
R/O	Spleen	0.65 ± 0.19

Source of PKH26⁺ Cells	Tissue Examined	% PKH26⁺ Cells Recovered^*
FR25Lin⁻	BM	12.75 ± 1.0
FR25Lin⁻	Spleen	0.27 ± 0.02
R/O	BM	1.25 ± 0.55
R/O	Spleen	0.65 ± 0.19

Primary B6D2F₁ lethally irradiated female mice received 2.5 × 10⁶ male FR25Lin⁻ or R/O cells labeled with PKH26. Forty-eight hours later, tibias and femurs, as well as spleen, were harvested and cell sorted for PKH26 bright cells.

% recovery = \frac{# of PKH bright cells recovered}{# of PKH bright cells injected}

The values represent the mean ± SEM for five experiments. Fr25lin⁻ cells are found in significantly larger numbers (P < .001) in marrow compared with spleen.

This is calculated as the % number of labeled cells × the total number of cells in the hind limb bones divided by the 8.0 ± 0.5% of the whole marrow that these bones represent (total skeletal marrow) or the whole spleen.

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