Production of GM-CSF and G-CSF In Vitro by Peritoneal Cells From βc+/+ and βc−/− Mice
| Stimulus . | βc−/− . | βc+/+ . | ||
|---|---|---|---|---|
| GM-CSF . | G-CSF . | GM-CSF . | G-CSF . | |
| Casein (Glaxo) | 21.3 ± 9.2 | 1.5 ± 0 | 13.3 ± 4.6 | 1.8 ± 1.1 |
| Casein (Sigma) | 16.0 ± 0.0 | 1.3 ± 0.4 | 13.3 ± 4.6 | 1.1 ± 0.6 |
| MTPBS | 0.9 ± 1.0 | 0.1 ± 0.06 | 1.1 ± 0.9 | 0.2 ± 0.06 |
| Stimulus . | βc−/− . | βc+/+ . | ||
|---|---|---|---|---|
| GM-CSF . | G-CSF . | GM-CSF . | G-CSF . | |
| Casein (Glaxo) | 21.3 ± 9.2 | 1.5 ± 0 | 13.3 ± 4.6 | 1.8 ± 1.1 |
| Casein (Sigma) | 16.0 ± 0.0 | 1.3 ± 0.4 | 13.3 ± 4.6 | 1.1 ± 0.6 |
| MTPBS | 0.9 ± 1.0 | 0.1 ± 0.06 | 1.1 ± 0.9 | 0.2 ± 0.06 |
A total of 1 × 106 peritoneal cells were incubated for 3 hours in 1 mL DMEM containing 10% newborn calf serum. To replicate cultures, 0.1 mL of 2% casein or 0.1 mL MTPBS was added. Values shown are mean values of CSF concentrations in nanograms per milliliter ± SD from assays on three replicate cultures using both male and female mice.