Effects of Retinoic Acid Antagonists on CD437-Dependent Apoptosis and Caspase Activation
| Treatment . | No. Viable Cells ×1,000 (% viability) . | Apoptotic Index (%) . | DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units) . |
|---|---|---|---|
| Control | 518 ± 50 | 4.8 ± 2.0 | 8.5 ± 0.3 |
| (76 ± 5) | |||
| CD437 | 439 ± 24 | 52.4 ± 16.03-150 | 18.1 ± 0.83-150 |
| (68 ± 1) | |||
| CD3106 | 542 ± 43 | 3.8 ± 1.3 | 7.1 ± 0.2 |
| (75 ± 5) | |||
| CD2665 | 577 ± 55 | 4.0 ± 1.9 | 8.0 ± 1.7 |
| (81 ± 5) | |||
| CD3159 | 506 ± 53 | 11.9 ± 3.9 | 8.2 ± 1.3 |
| (77 ± 6) | |||
| CD437 + CD3106 | 448 ± 49 | 40.5 ± 13.33-150 | 17.7 ± 2.33-150 |
| (75 ± 7) | |||
| CD437 + CD2665 | 420 ± 20 | 44.8 ± 9.93-150 | 20.3 ± 1.33-150 |
| (74 ± 2) | |||
| CD437 + CD3159 | 440 ± 29 | 47.3 ± 3.33-150 | 22.2 ± 2.53-150 |
| (67 ± 3) |
| Treatment . | No. Viable Cells ×1,000 (% viability) . | Apoptotic Index (%) . | DEVD-amc Hydrolytic Activity (Fluorescence Arbitrary Units) . |
|---|---|---|---|
| Control | 518 ± 50 | 4.8 ± 2.0 | 8.5 ± 0.3 |
| (76 ± 5) | |||
| CD437 | 439 ± 24 | 52.4 ± 16.03-150 | 18.1 ± 0.83-150 |
| (68 ± 1) | |||
| CD3106 | 542 ± 43 | 3.8 ± 1.3 | 7.1 ± 0.2 |
| (75 ± 5) | |||
| CD2665 | 577 ± 55 | 4.0 ± 1.9 | 8.0 ± 1.7 |
| (81 ± 5) | |||
| CD3159 | 506 ± 53 | 11.9 ± 3.9 | 8.2 ± 1.3 |
| (77 ± 6) | |||
| CD437 + CD3106 | 448 ± 49 | 40.5 ± 13.33-150 | 17.7 ± 2.33-150 |
| (75 ± 7) | |||
| CD437 + CD2665 | 420 ± 20 | 44.8 ± 9.93-150 | 20.3 ± 1.33-150 |
| (74 ± 2) | |||
| CD437 + CD3159 | 440 ± 29 | 47.3 ± 3.33-150 | 22.2 ± 2.53-150 |
| (67 ± 3) |
Cells (500,000/mL) were treated with CD437 (1 × 10−6 mol/L), CD3106 (1 × 10−5mol/L), CD2665 (1 × 10−5 mol/L), CD3159 (1 × 10−5 mol/L), or CD437 in combination with each of the other three compounds for 6 hours. At the end of treatment, aliquots of the cultures were stained with erythrosin for the determination of the number of cells and their viability, and with DAPI for the determination of the number of apoptotic cells. Remaining cells were obtained, homogenized, and the extract was assayed for DEVD-amc hydrolytic activity. Each value represents the mean ± SD of three replicate cultures.
Statistically higher than control following the Student’st-test (P < .01).