Table 1. Screening of the ( / - mi / mi... | American Society of Hematology

Table 1.

Screening of the (+/+-mi/mi) and (+/+-tg/tg) Subtracted cDNA Libraries by Southern Analyses

Subtracted cDNA Libraries	No. of Clones Fractioned on the Gel	No. of Clones Yielding Hybridization Signals With +/+-Probe	No. of Clones Yielding Stronger Signals With +/+-Probe Than With mi/mi- ortg/tg-Probe	No. of Clones Yielding Stronger Signals With mi/mi- ortg/tg-Probe Than With +/+-Probe
Experiment no. 1
+/+-mi/mi	80	44	5	0
+/+-tg/tg	80	46	1	0
Experiment no. 2
+/+-mi/mi	80	35	3	1
+/+-tg/tg	80	40	1	0
Experiment no. 3
+/+-mi/mi	80	38	4	0
+/+-tg/tg	80	39	0	1
Experiment no. 4
+/+-mi/mi	80	41	5	0
+/+-tg/tg	80	37	2	0
Experiment no. 5
+/+-mi/mi	80	40	3	0
+/+-tg/tg	80	43	1	0
Average
+/+-mi/mi	80	39.6 ± 1.5	4.0 ± 0.4	0.2 ± 0.2
+/+-tg/tg	80	41.0 ± 1.6	1.0 ± 0.3^*	0.2 ± 0.2
Total
+/+-mi/mi	400	198	20	1
+/+-tg/tg	400	205	5^†	1

Subtracted cDNA Libraries	No. of Clones Fractioned on the Gel	No. of Clones Yielding Hybridization Signals With +/+-Probe	No. of Clones Yielding Stronger Signals With +/+-Probe Than With mi/mi- ortg/tg-Probe	No. of Clones Yielding Stronger Signals With mi/mi- ortg/tg-Probe Than With +/+-Probe
Experiment no. 1
+/+-mi/mi	80	44	5	0
+/+-tg/tg	80	46	1	0
Experiment no. 2
+/+-mi/mi	80	35	3	1
+/+-tg/tg	80	40	1	0
Experiment no. 3
+/+-mi/mi	80	38	4	0
+/+-tg/tg	80	39	0	1
Experiment no. 4
+/+-mi/mi	80	41	5	0
+/+-tg/tg	80	37	2	0
Experiment no. 5
+/+-mi/mi	80	40	3	0
+/+-tg/tg	80	43	1	0
Average
+/+-mi/mi	80	39.6 ± 1.5	4.0 ± 0.4	0.2 ± 0.2
+/+-tg/tg	80	41.0 ± 1.6	1.0 ± 0.3^*	0.2 ± 0.2
Total
+/+-mi/mi	400	198	20	1
+/+-tg/tg	400	205	5^†	1

The result of experiment no. 1 is shown in Fig. 2.

P < .01 by t-test when compared with the value of the (+/+-mi/mi) library.

P < .01 by χ²-test when compared with the value of the (+/+-mi/mi) library.