Inhibition of IL-6–Induced Macrophage Differentiation of 1A9-M Cells by a Dominant-Negative Form of Stat3, But Not by Constitutively Expressed BCL2
| Transfectant . | Increased Median Fluorescence . | Morphological Change Into Macrophages (%) . | |
|---|---|---|---|
| F4/80 . | CD32 . | ||
| Parent 1A9-M | 34.0 ± 1.87 | 248.1 ± 11.98 | 85.0 ± 3.68 |
| 1A9-M/pCAGGS clone 1 | 49.6 ± 5.51 | 304.1 ± 14.30 | 84.5 ± 5.64 |
| 1A9-M/pCAGGS clone 2 | 34.7 ± 3.85 | 242.3 ± 4.53 | 84.7 ± 3.74 |
| 1A9-M/pCAGGS clone 3 | 31.9 ± 1.70 | 220.2 ± 10.99 | 83.0 ± 3.40 |
| 1A9-M/ST3D clone 1 | 0.3 ± 0.33 | 5.6 ± 1.70 | 1.8 ± 0.82 |
| 1A9-M/ST3D clone 2 | 0.3 ± 0.21 | 7.3 ± 0.98 | 3.0 ± 1.25 |
| 1A9-M/ST3D clone A | 8.6 ± 1.03 | 75.1 ± 7.35 | 48.8 ± 5.24 |
| 1A9-M/ST3D clone B | 0.5 ± 0.45 | 8.2 ± 1.07 | 4.7 ± 2.82 |
| 1A9-M/BCL2 clone 1 | 36.9 ± 2.67 | 225.1 ± 8.26 | 81.8 ± 4.78 |
| 1A9-M/BCL2 clone 2 | 36.6 ± 4.13 | 220.9 ± 17.90 | 84.5 ± 4.50 |
| 1A9-M/BCL2 clone 3 | 32.4 ± 2.09 | 260.4 ± 9.66 | 88.8 ± 3.74 |
| Transfectant . | Increased Median Fluorescence . | Morphological Change Into Macrophages (%) . | |
|---|---|---|---|
| F4/80 . | CD32 . | ||
| Parent 1A9-M | 34.0 ± 1.87 | 248.1 ± 11.98 | 85.0 ± 3.68 |
| 1A9-M/pCAGGS clone 1 | 49.6 ± 5.51 | 304.1 ± 14.30 | 84.5 ± 5.64 |
| 1A9-M/pCAGGS clone 2 | 34.7 ± 3.85 | 242.3 ± 4.53 | 84.7 ± 3.74 |
| 1A9-M/pCAGGS clone 3 | 31.9 ± 1.70 | 220.2 ± 10.99 | 83.0 ± 3.40 |
| 1A9-M/ST3D clone 1 | 0.3 ± 0.33 | 5.6 ± 1.70 | 1.8 ± 0.82 |
| 1A9-M/ST3D clone 2 | 0.3 ± 0.21 | 7.3 ± 0.98 | 3.0 ± 1.25 |
| 1A9-M/ST3D clone A | 8.6 ± 1.03 | 75.1 ± 7.35 | 48.8 ± 5.24 |
| 1A9-M/ST3D clone B | 0.5 ± 0.45 | 8.2 ± 1.07 | 4.7 ± 2.82 |
| 1A9-M/BCL2 clone 1 | 36.9 ± 2.67 | 225.1 ± 8.26 | 81.8 ± 4.78 |
| 1A9-M/BCL2 clone 2 | 36.6 ± 4.13 | 220.9 ± 17.90 | 84.5 ± 4.50 |
| 1A9-M/BCL2 clone 3 | 32.4 ± 2.09 | 260.4 ± 9.66 | 88.8 ± 3.74 |
1A9-M/pCAGGS (clone 1-3), 1A9-M/ST3D (clone 1, 2, A, B), 1A9-M/BCL2 (clone 1-3), and parent 1A9-M cells were cultured with or without 20 ng/mL IL-6 for 48 hours. Surface expression of CD32/CD16 and a F4/80 antigen was evaluated by flow cytometry. To determine morphological changes into macrophages, cultured cells were stained with May-Grunwald Giemsa. The increase of cells expressing F4/80 antigen or CD32 and morphologically macrophage-like cells by IL-6 treatment is shown as the mean ± SD of triplicate cultures.