Relative Number of Flk-1+ Cell Clusters on OP9 Feeder Layer
| mFlt-1hIgG1 . | VEGF . | PIGF . | ||||
|---|---|---|---|---|---|---|
| 0 . | 1 . | 3 . | 10 . | 50 . | 100 . | |
| 0 | 100 | 226 ± 18 | 234 ± 15 | 234 ± 21 | 199 ± 10 | 115 ± 14 |
| 100 | 10 ± 4 | 42 ± 20 | 195 ± 18 | 201 ± 19 | 185 ± 14 | 27 ± 10 |
| 500 | 14 ± 0 | 19 ± 3 | 18 ± 7 | 196 ± 21 | 183 ± 25 | 17 ± 2 |
| mFlt-1hIgG1 . | VEGF . | PIGF . | ||||
|---|---|---|---|---|---|---|
| 0 . | 1 . | 3 . | 10 . | 50 . | 100 . | |
| 0 | 100 | 226 ± 18 | 234 ± 15 | 234 ± 21 | 199 ± 10 | 115 ± 14 |
| 100 | 10 ± 4 | 42 ± 20 | 195 ± 18 | 201 ± 19 | 185 ± 14 | 27 ± 10 |
| 500 | 14 ± 0 | 19 ± 3 | 18 ± 7 | 196 ± 21 | 183 ± 25 | 17 ± 2 |
Varying concentrations (in nanograms per milliliter) of mFlt-1-hIgG1 and/or its ligands were added to cultures of Flk-1+ cells on OP9 feeder layer. After 3 days of culture, the number of Flk-1+ cell clusters in each culture was counted. The results are expressed as a percentage of that in culture without exogenous factors. Values are the mean (±SD) of three independent experiments.